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Literature summary for 2.4.1.249 extracted from

  • Kogawa, K.; Kato, N.; Kazuma, K.; Noda, N.; Suzuki, M.
    Purification and characterization of UDP-glucose: anthocyanin 3',5'-O-glucosyltransferase from Clitoria ternatea (2007), Planta, 226, 1501-1509.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diethyldicarbonate 10 mM, 16% activity retains Clitoria ternatea
dithioerythritol 10 mM, 83% activity retains Clitoria ternatea
iodoacetate 10 mM, 84% activity retains Clitoria ternatea
N-ethylmaleimide 10 mM, 16% activity retains Clitoria ternatea
p-chloromercuribenzoic acid 10 mM, completely inhibited Clitoria ternatea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0389
-
delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside
-
Clitoria ternatea
0.138
-
delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside
-
Clitoria ternatea
1.49
-
UDP-D-glucose apparent, delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as saturated substrate Clitoria ternatea
6.18
-
UDP-D-glucose apparent, delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside as saturated substrate Clitoria ternatea

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 10 mM, 82% activity retains Clitoria ternatea
EDTA 10 mM, 75% activity retains Clitoria ternatea
FeCl2 10 mM, 73% activity retains Clitoria ternatea
MgCl2 10 mM, 88% activity retains Clitoria ternatea
additional information completely inhibited by 10 mM CuCl2 Clitoria ternatea
ZnCl2 10 mM, 62% activity retains Clitoria ternatea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
gel filtration Clitoria ternatea
50800
-
SDS-PAGE Clitoria ternatea
58000
-
1 * 58000, SDS-PAGE Clitoria ternatea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside Clitoria ternatea 3'-O-glucosyltransferase activity, first reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside
-
?
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside Clitoria ternatea 5'-O-glucosyltransferase activity, second reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside
-
?

Organism

Organism UniProt Comment Textmining
Clitoria ternatea A4F1Q6 gene An3'5'GT; butterfly pea
-

Purification (Commentary)

Purification (Comment) Organism
from petals, which accumulate polyacylated anthocyanins and ternatins, using ammonium sulfate precipitation, DEAE Toyopearl, Reactive yellow 86, Cellufine HAP, and Mono Q chromatography, UA5'GT: 1007fold purified, UA3'GT: 346 fold purified Clitoria ternatea

Reaction

Reaction Comment Organism Reaction ID
UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside = UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase has both, UA3'GT and UA5'GT activities, highly regioselective to the 3' and 5' OH groups Clitoria ternatea
UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside = UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3',5'-di-O-beta-D-glucoside UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase has both, UA3'GT and UA5'GT activities, highly regioselective to the 3' and 5' OH groups Clitoria ternatea

Source Tissue

Source Tissue Comment Organism Textmining
petal
-
Clitoria ternatea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000276
-
UA5'GT activity, substrate: delphinidin 3,3'-di-O-beta-D-glucoside Clitoria ternatea
0.000324
-
UA3'GT activity, substrate: delphinidin 3-O-beta-D-glucoside Clitoria ternatea
0.002
-
UA3'GT activity, substrate: cyanidin 3-O-(6''-O-malonyl)-beta-D-glucoside Clitoria ternatea
0.01
-
UA5'GT activity, substrate: petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside Clitoria ternatea
0.98
-
UA3'GT activity, substrate: delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside Clitoria ternatea
2.29
-
UA5'GT activity, substrate: delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside Clitoria ternatea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity detected with cyanidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside, pelargonidin 3-O-(6''-O-malonyl)-beta-D-glucoside, delphinidin 3,5-di-O-beta-D-glucoside. No activity with delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside using UDP-D-galactose as sugar donor Clitoria ternatea ?
-
?
UDP-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
Clitoria ternatea UDP + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside 3'-O-beta-D-glucoside 24.3% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate ?
UDP-D-glucose + delphinidin 3,3'-di-O-beta-D-glucoside 3.3% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate Clitoria ternatea UDP + delphinidin 3,3',5'-tri-O-beta-D-glucoside
-
?
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside 3'-O-glucosyltransferase activity, first reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner Clitoria ternatea UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside
-
?
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside 3'-O-glucosyltransferase activity, catalyzed by UA3'5'GT Clitoria ternatea UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside
-
?
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside 5'-O-glucosyltransferase activity, second reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner Clitoria ternatea UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside
-
?
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside 5'-O-glucosyltransferase activity, catalyzed by UA3'5'GT Clitoria ternatea UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside
-
?
UDP-D-glucose + delphinidin 3-O-beta-D-glucoside 3.9% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate Clitoria ternatea UDP + delphinidin 3,3'-di-O-beta-D-glucoside
-
?
UDP-D-glucose + petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside
-
Clitoria ternatea UDP + petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside 5'-O-beta-D-glucoside 123% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate ?

Subunits

Subunits Comment Organism
monomer 1 * 58000, SDS-PAGE Clitoria ternatea

Synonyms

Synonyms Comment Organism
UA3'5'GT
-
Clitoria ternatea
UA3'GT
-
Clitoria ternatea
UA5'GT
-
Clitoria ternatea
UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase
-
Clitoria ternatea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside UDP-glucose as sugar donor Clitoria ternatea
4.9
-
UDP-D-glucose delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside as substrate Clitoria ternatea
5.1
-
UDP-D-glucose delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate Clitoria ternatea
5.4
-
delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside UDP-glucose as sugar donor Clitoria ternatea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 pH 7.5 and pH 8.0 in potassium phosphate buffer and Tris-HCl buffer, respectively Clitoria ternatea