Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | 10 mM, 16% activity retains | Clitoria ternatea | |
dithioerythritol | 10 mM, 83% activity retains | Clitoria ternatea | |
iodoacetate | 10 mM, 84% activity retains | Clitoria ternatea | |
N-ethylmaleimide | 10 mM, 16% activity retains | Clitoria ternatea | |
p-chloromercuribenzoic acid | 10 mM, completely inhibited | Clitoria ternatea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0389 | - |
delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside | - |
Clitoria ternatea | |
0.138 | - |
delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside | - |
Clitoria ternatea | |
1.49 | - |
UDP-D-glucose | apparent, delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as saturated substrate | Clitoria ternatea | |
6.18 | - |
UDP-D-glucose | apparent, delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside as saturated substrate | Clitoria ternatea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 10 mM, 82% activity retains | Clitoria ternatea | |
EDTA | 10 mM, 75% activity retains | Clitoria ternatea | |
FeCl2 | 10 mM, 73% activity retains | Clitoria ternatea | |
MgCl2 | 10 mM, 88% activity retains | Clitoria ternatea | |
additional information | completely inhibited by 10 mM CuCl2 | Clitoria ternatea | |
ZnCl2 | 10 mM, 62% activity retains | Clitoria ternatea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Clitoria ternatea |
50800 | - |
SDS-PAGE | Clitoria ternatea |
58000 | - |
1 * 58000, SDS-PAGE | Clitoria ternatea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside | Clitoria ternatea | 3'-O-glucosyltransferase activity, first reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner | UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside | - |
? | |
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside | Clitoria ternatea | 5'-O-glucosyltransferase activity, second reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner | UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clitoria ternatea | A4F1Q6 | gene An3'5'GT; butterfly pea | - |
Purification (Comment) | Organism |
---|---|
from petals, which accumulate polyacylated anthocyanins and ternatins, using ammonium sulfate precipitation, DEAE Toyopearl, Reactive yellow 86, Cellufine HAP, and Mono Q chromatography, UA5'GT: 1007fold purified, UA3'GT: 346 fold purified | Clitoria ternatea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside = UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside | UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase has both, UA3'GT and UA5'GT activities, highly regioselective to the 3' and 5' OH groups | Clitoria ternatea | |
UDP-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside = UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3',5'-di-O-beta-D-glucoside | UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase has both, UA3'GT and UA5'GT activities, highly regioselective to the 3' and 5' OH groups | Clitoria ternatea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
petal | - |
Clitoria ternatea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000276 | - |
UA5'GT activity, substrate: delphinidin 3,3'-di-O-beta-D-glucoside | Clitoria ternatea |
0.000324 | - |
UA3'GT activity, substrate: delphinidin 3-O-beta-D-glucoside | Clitoria ternatea |
0.002 | - |
UA3'GT activity, substrate: cyanidin 3-O-(6''-O-malonyl)-beta-D-glucoside | Clitoria ternatea |
0.01 | - |
UA5'GT activity, substrate: petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside | Clitoria ternatea |
0.98 | - |
UA3'GT activity, substrate: delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside | Clitoria ternatea |
2.29 | - |
UA5'GT activity, substrate: delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside | Clitoria ternatea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity detected with cyanidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-D-glucoside, pelargonidin 3-O-(6''-O-malonyl)-beta-D-glucoside, delphinidin 3,5-di-O-beta-D-glucoside. No activity with delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside using UDP-D-galactose as sugar donor | Clitoria ternatea | ? | - |
? | |
UDP-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside | - |
Clitoria ternatea | UDP + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside 3'-O-beta-D-glucoside | 24.3% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate | ? | |
UDP-D-glucose + delphinidin 3,3'-di-O-beta-D-glucoside | 3.3% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate | Clitoria ternatea | UDP + delphinidin 3,3',5'-tri-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside | 3'-O-glucosyltransferase activity, first reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner | Clitoria ternatea | UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside | - |
? | |
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside | 3'-O-glucosyltransferase activity, catalyzed by UA3'5'GT | Clitoria ternatea | UDP + delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside | - |
? | |
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside | 5'-O-glucosyltransferase activity, second reaction of UA3'5'GT in the biosynthesis of ternatins, transferring two glucosyl groups in a stepwise manner | Clitoria ternatea | UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3'-O-beta-D-glucoside | 5'-O-glucosyltransferase activity, catalyzed by UA3'5'GT | Clitoria ternatea | UDP + delphinidin 3-O-(6''-O-malonyl)-beta-glucoside-3',5'-di-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + delphinidin 3-O-beta-D-glucoside | 3.9% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate | Clitoria ternatea | UDP + delphinidin 3,3'-di-O-beta-D-glucoside | - |
? | |
UDP-D-glucose + petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside | - |
Clitoria ternatea | UDP + petunidin 3-O-(6''-O-malonyl)-beta-D-glucoside 5'-O-beta-D-glucoside | 123% relative activity compared to delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Clitoria ternatea |
Synonyms | Comment | Organism |
---|---|---|
UA3'5'GT | - |
Clitoria ternatea |
UA3'GT | - |
Clitoria ternatea |
UA5'GT | - |
Clitoria ternatea |
UDP-glucose:anthocyanin 3',5'-O-glucosyltransferase | - |
Clitoria ternatea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside | UDP-glucose as sugar donor | Clitoria ternatea | |
4.9 | - |
UDP-D-glucose | delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside as substrate | Clitoria ternatea | |
5.1 | - |
UDP-D-glucose | delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside as substrate | Clitoria ternatea | |
5.4 | - |
delphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3'-O-beta-glucoside | UDP-glucose as sugar donor | Clitoria ternatea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | pH 7.5 and pH 8.0 in potassium phosphate buffer and Tris-HCl buffer, respectively | Clitoria ternatea |