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Literature summary for 2.4.1.248 extracted from

  • Yang, S.J.; Choi, S.J.; Park, B.R.; Kim, Y.M.
    Thermostable CITase from Thermoanaerobacter thermocopriae shows negative cooperativity (2019), Biotechnol. Lett., 41, 625-632 .
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme can be applied to produce relatively large CIs at high temperature Thermoanaerobacter thermocopriae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermoanaerobacter thermocopriae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
171000
-
SDS-PAGE Thermoanaerobacter thermocopriae
175269
-
calculated from sequence Thermoanaerobacter thermocopriae

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter thermocopriae WP_028992696
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoanaerobacter thermocopriae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran cycloisomaltooligosaccharide-7 to cycloisomaltooligosaccharide-20 are mainly produced, with CI-7 as the main product Thermoanaerobacter thermocopriae ?
-
?

Subunits

Subunits Comment Organism
? x * 171000, SDS-PAGE Thermoanaerobacter thermocopriae
? x * 175269, calculated from sequence Thermoanaerobacter thermocopriae

Synonyms

Synonyms Comment Organism
CITase
-
Thermoanaerobacter thermocopriae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
1 h incubation Thermoanaerobacter thermocopriae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 40% of maximal activity, 70°C: about 65% of maximal activity Thermoanaerobacter thermocopriae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
24 h, thermal stability of the protein is 63%. NO loss of activity after 1 h Thermoanaerobacter thermocopriae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Thermoanaerobacter thermocopriae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 pH 5.5: about 75% of maximal activity, pH 7.0: about 75% of maximal activity Thermoanaerobacter thermocopriae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 4°C, 18 h, stable Thermoanaerobacter thermocopriae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
67.4
-
Dextran pH 6.0, 50°C Thermoanaerobacter thermocopriae