Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be applied to produce relatively large CIs at high temperature | Thermoanaerobacter thermocopriae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermoanaerobacter thermocopriae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
171000 | - |
SDS-PAGE | Thermoanaerobacter thermocopriae |
175269 | - |
calculated from sequence | Thermoanaerobacter thermocopriae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter thermocopriae | WP_028992696 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacter thermocopriae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran | cycloisomaltooligosaccharide-7 to cycloisomaltooligosaccharide-20 are mainly produced, with CI-7 as the main product | Thermoanaerobacter thermocopriae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 171000, SDS-PAGE | Thermoanaerobacter thermocopriae |
? | x * 175269, calculated from sequence | Thermoanaerobacter thermocopriae |
Synonyms | Comment | Organism |
---|---|---|
CITase | - |
Thermoanaerobacter thermocopriae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h incubation | Thermoanaerobacter thermocopriae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 40°C: about 40% of maximal activity, 70°C: about 65% of maximal activity | Thermoanaerobacter thermocopriae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
24 h, thermal stability of the protein is 63%. NO loss of activity after 1 h | Thermoanaerobacter thermocopriae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermoanaerobacter thermocopriae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | pH 5.5: about 75% of maximal activity, pH 7.0: about 75% of maximal activity | Thermoanaerobacter thermocopriae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 4°C, 18 h, stable | Thermoanaerobacter thermocopriae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67.4 | - |
Dextran | pH 6.0, 50°C | Thermoanaerobacter thermocopriae |