Cloned (Comment) | Organism |
---|---|
GBSSI, DNA sequence determination and analysis, expression in Escherichia coli, complementation of the sta2-1 mutation, phylogenetic tree | Chlamydomonas reinhardtii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the sta2-29::ARG7 mutation causes loss of GBSSI activity, the Sta2-1 mutation results in production of a truncated 58 kDa GBSSI isozyme | Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE | Chlamydomonas reinhardtii |
69000 | - |
x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE | Chlamydomonas reinhardtii |
76000 | - |
x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + (1,4-alpha-glucosyl)n | Chlamydomonas reinhardtii | i.e. starch, composition of starch granule, including amylopectin, amylose, A-type and B-type crystals | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
additional information | Chlamydomonas reinhardtii | decreased amylose synthesis activity and altered starch granule morphology during nitrogen starvation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | O64925 | complete cDNA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the transit peptide of the immature isozyme GBSSI is cleaved off resulting in the mature enzyme | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + (1,4-alpha-glucosyl)n | i.e. starch, composition of starch granule, including amylopectin, amylose, A-type and B-type crystals | Chlamydomonas reinhardtii | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
ADP-glucose + (1,4-alpha-glucosyl)n | i.e. starch | Chlamydomonas reinhardtii | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
additional information | decreased amylose synthesis activity and altered starch granule morphology during nitrogen starvation, overview | Chlamydomonas reinhardtii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
GBSSI | - |
Chlamydomonas reinhardtii |
granule-bound starch synthase I | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Chlamydomonas reinhardtii |