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Literature summary for 2.4.1.238 extracted from

  • Fukuchi-Mizutani, M.; Okuhara, H.; Fukui, Y.; Nakao, M.; Katsumoto, Y.; Yonekura-Sakakibara, K.; Kusumi, T.; Hase, T.; Tanaka, Y.
    Biochemical and molecular characterization of a novel UDP-glucose:anthocyanin 3'-O-glucosyltransferase, a key enzyme for blue anthocyanin biosynthesis, from gentian (2003), Plant Physiol., 132, 1652-1663.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
agriculture 3'GT cDNA is a useful molecular tool for modification of flower color using genetic engineering, production of blue anthocyanins Gentiana triflora

Cloned(Commentary)

Cloned (Comment) Organism
expression in transgenic Petunia hybrida and in Escherichia coli JM105, sequencing Gentiana triflora

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 80% inhibition Gentiana triflora
Co2+ 69% inhibition Gentiana triflora
additional information not inhibited by Mg2+, Ca2+, Mn2+, EDTA or EGTA Gentiana triflora
UDP competitive inhibition Gentiana triflora
Zn2+ 26% inhibition Gentiana triflora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
delphinidin 3,5-diglucoside pH 8.5, 30°C, recombinant 3'GT Gentiana triflora

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant 3'GT expressed in Escherichia coli JM105 Gentiana triflora
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54037
-
x * 55000, SDS-PAGE, x * 54037, sequence calculation Gentiana triflora
55000
-
x * 55000, SDS-PAGE, x * 54037, sequence calculation Gentiana triflora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gentiana triflora key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin ?
-
?

Organism

Organism UniProt Comment Textmining
Gentiana triflora Q8H0F2 var. japonica
-

Purification (Commentary)

Purification (Comment) Organism
native 3'GT from petals, recombinant 3'GT expressed in Escherichia coli JM105 Gentiana triflora

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside this enzyme specifically glucosylates the 3'-hydroxy group of delphinidin-3-O-glucosyl-5-O-(6-O-caffeoylglucosyl)-3'-O-(6-O-caffeoylglucoside)-type anthocyanins containing glucose groups at the 3 and 5 positions, acts on delphinidin 3,5-di-O-glucoside in gentian, Gentiana triflora Gentiana triflora

Source Tissue

Source Tissue Comment Organism Textmining
additional information 3'GT mRNA is barely detectable in leaves Gentiana triflora
-
petal blue, from flowers at a pre-anthesis stage, 3'GT mRNA is abundant in petals at the first three developmental stages, but dramatically decreased in fully opened flowers Gentiana triflora
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin Gentiana triflora ?
-
?
additional information no sugar donors: UDP-galactose, UDP-GlcUA, UDP-N-acetylgalactosamine, not: pelargonidin 3,5-diglucoside, cyanidin 3,5-diglucoside, dihydroflavonols, dihydrokaempferol, dihydroquercetin, dihydromyricetin, flavone, tricetin Gentiana triflora ?
-
?
UDP-alpha-D-glucose + delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) in vitro, 50.9% of the activity with delphinidin 3,5-diglucoside Gentiana triflora UDP + delphinidin 3-O,3'-O-di-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
-
?
UDP-glucose + an anthocyanidin strict substrate specificity, 3'GT specifically glucosylates the 3'-hydroxy group of delphinidin-type anthocyanins containing glucose groups at the 3 and 5 positions and specifically uses UDP-glucose as the sugar donor, 3’GT strictly requires three hydroxy groups on the B-ring for its substrates Gentiana triflora UDP + an anthocyanidin 3'-O-beta-D-glucoside
-
?
UDP-glucose + delphinidin poor substrate, 3.3% of the activity with delphinidin 3,5-diglucoside Gentiana triflora UDP + delphinidin 3'-glucoside
-
?
UDP-glucose + delphinidin 3,5-di-O-beta-D-glucoside 3'GT glucosylates the 3'-hydroxy group of delphinidin 3,5-diglucoside, 2fold higher activity than with delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) as substrate Gentiana triflora UDP + delphinidin 3,5,7-tri-O-beta-D-glucoside
-
?
UDP-glucose + delphinidin 3-glucoside poor substrate, 2.9% of the activity with delphinidin 3,5-diglucoside Gentiana triflora UDP + delphinidin 3,3'-diglucoside
-
?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE, x * 54037, sequence calculation Gentiana triflora

Synonyms

Synonyms Comment Organism
3'GT
-
Gentiana triflora
UDP-glucose:anthocyanin 3'-O-glucosyltransferase
-
Gentiana triflora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Gentiana triflora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Gentiana triflora

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.76
-
UDP pH 8.5, 30°C, recombinant 3'GT Gentiana triflora