Application | Comment | Organism |
---|---|---|
agriculture | 3'GT cDNA is a useful molecular tool for modification of flower color using genetic engineering, production of blue anthocyanins | Gentiana triflora |
Cloned (Comment) | Organism |
---|---|
expression in transgenic Petunia hybrida and in Escherichia coli JM105, sequencing | Gentiana triflora |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 80% inhibition | Gentiana triflora | |
Co2+ | 69% inhibition | Gentiana triflora | |
additional information | not inhibited by Mg2+, Ca2+, Mn2+, EDTA or EGTA | Gentiana triflora | |
UDP | competitive inhibition | Gentiana triflora | |
Zn2+ | 26% inhibition | Gentiana triflora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
delphinidin 3,5-diglucoside | pH 8.5, 30°C, recombinant 3'GT | Gentiana triflora |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | recombinant 3'GT expressed in Escherichia coli JM105 | Gentiana triflora | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54037 | - |
x * 55000, SDS-PAGE, x * 54037, sequence calculation | Gentiana triflora |
55000 | - |
x * 55000, SDS-PAGE, x * 54037, sequence calculation | Gentiana triflora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gentiana triflora | key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gentiana triflora | Q8H0F2 | var. japonica | - |
Purification (Comment) | Organism |
---|---|
native 3'GT from petals, recombinant 3'GT expressed in Escherichia coli JM105 | Gentiana triflora |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside | this enzyme specifically glucosylates the 3'-hydroxy group of delphinidin-3-O-glucosyl-5-O-(6-O-caffeoylglucosyl)-3'-O-(6-O-caffeoylglucoside)-type anthocyanins containing glucose groups at the 3 and 5 positions, acts on delphinidin 3,5-di-O-glucoside in gentian, Gentiana triflora | Gentiana triflora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | 3'GT mRNA is barely detectable in leaves | Gentiana triflora | - |
petal | blue, from flowers at a pre-anthesis stage, 3'GT mRNA is abundant in petals at the first three developmental stages, but dramatically decreased in fully opened flowers | Gentiana triflora | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin | Gentiana triflora | ? | - |
? | |
additional information | no sugar donors: UDP-galactose, UDP-GlcUA, UDP-N-acetylgalactosamine, not: pelargonidin 3,5-diglucoside, cyanidin 3,5-diglucoside, dihydroflavonols, dihydrokaempferol, dihydroquercetin, dihydromyricetin, flavone, tricetin | Gentiana triflora | ? | - |
? | |
UDP-alpha-D-glucose + delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) | in vitro, 50.9% of the activity with delphinidin 3,5-diglucoside | Gentiana triflora | UDP + delphinidin 3-O,3'-O-di-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside) | - |
? | |
UDP-glucose + an anthocyanidin | strict substrate specificity, 3'GT specifically glucosylates the 3'-hydroxy group of delphinidin-type anthocyanins containing glucose groups at the 3 and 5 positions and specifically uses UDP-glucose as the sugar donor, 3GT strictly requires three hydroxy groups on the B-ring for its substrates | Gentiana triflora | UDP + an anthocyanidin 3'-O-beta-D-glucoside | - |
? | |
UDP-glucose + delphinidin | poor substrate, 3.3% of the activity with delphinidin 3,5-diglucoside | Gentiana triflora | UDP + delphinidin 3'-glucoside | - |
? | |
UDP-glucose + delphinidin 3,5-di-O-beta-D-glucoside | 3'GT glucosylates the 3'-hydroxy group of delphinidin 3,5-diglucoside, 2fold higher activity than with delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) as substrate | Gentiana triflora | UDP + delphinidin 3,5,7-tri-O-beta-D-glucoside | - |
? | |
UDP-glucose + delphinidin 3-glucoside | poor substrate, 2.9% of the activity with delphinidin 3,5-diglucoside | Gentiana triflora | UDP + delphinidin 3,3'-diglucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE, x * 54037, sequence calculation | Gentiana triflora |
Synonyms | Comment | Organism |
---|---|---|
3'GT | - |
Gentiana triflora |
UDP-glucose:anthocyanin 3'-O-glucosyltransferase | - |
Gentiana triflora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Gentiana triflora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Gentiana triflora |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
UDP | pH 8.5, 30°C, recombinant 3'GT | Gentiana triflora |