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Literature summary for 2.4.1.237 extracted from

  • Isayenkova, J.; Wray, V.; Nimtz, M.; Strack, D.; Vogt, T.
    Cloning and functional characterization of two regioselective flavonoid glucosyltransferases from Beta vulgaris (2006), Phytochemistry, 67, 1598-1612.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functionally expressed in Escherichia coli as His- and GST-tagged protein, UGT71F1 Beta vulgaris
functionally expressed in Escherichia coli as His- and GST-tagged protein, UGT73A4 Beta vulgaris

Inhibitors

Inhibitors Comment Organism Structure
4,4'-diisothiocyanostilbene-2,2'-disulfonic acid 0.01 mM, UGT71F1; 0.01 mM, UGT73A4 Beta vulgaris
Co2+ complete inhibition near 0.04 mM, UGT71F1; complete inhibition near 0.04 mM, UGT73A4 Beta vulgaris
Cu2+ complete inhibition near 0.04 mM, UGT71F1; complete inhibition near 0.04 mM, UGT73A4 Beta vulgaris
p-chloromercuribenzene sulfonic acid UGT71F1, 0.01mM, 22% inhibition; UGT73A4 activity is completely inhibited at 0.01mM Beta vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
quercetin UGT71F1 Beta vulgaris
0.011
-
quercetin UGT73A4 Beta vulgaris
0.0192
-
apigenin UGT73A4 Beta vulgaris
0.0268
-
apigenin UGT71F1 Beta vulgaris
0.029
-
betanidin UGT71F1 Beta vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for bivalent cations, UGT71F1 Beta vulgaris
additional information no requirement for bivalent cations, UGT73A4 Beta vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54070
-
calculated from sequence, UGT73A4 Beta vulgaris
54390
-
calculated from sequence, UGT71F1 Beta vulgaris

Organism

Organism UniProt Comment Textmining
Beta vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant UGT71F1 Beta vulgaris
recombinant UGT73A4 Beta vulgaris

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
UGT71F1 can not be frozen nor stored at 4°C as 50% activity is lost within 24 h Beta vulgaris
UGT73A4 can be stored at 4°C or frozen at -20°C or -80°C for several months without requiring addition of stabilizing agents such as bovine serum albumin or glycerol Beta vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + kaempferol 81% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + kaempferol 7-O-beta-D-glucoside
-
?
UDP-alpha-D-glucose + kaempferol 17% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + kaempferol 3-O-beta-D-glucoside
-
?
UDP-alpha-D-glucose + quercetin UGT71F1 Beta vulgaris UDP + quercetin 7-O-beta-D-glucoside + quercetin 3-O-beta-D-glucoside
-
?
UDP-alpha-D-glucose + quercetin 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 Beta vulgaris UDP + quercetin 7-O-beta-D-glucoside + quercetin 4'-O-beta-D-glucoside
-
?
UDP-glucose + apigenin 40% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + apigenin 7-O-beta-D-glucoside
-
?
UDP-glucose + apigenin 90% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + apigenin 7-O-beta-D-glucoside
-
?
UDP-glucose + betanidin 28% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + betanidin 5-O-beta-D-glucoside + betanidin 6-O-beta-D-glucoside
-
?
UDP-glucose + caffeic acid 90% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + caffeoyl 3-O-beta-D-glucoside
-
?
UDP-glucose + chrysin 70% of the activity with quercetinUGT73A4 Beta vulgaris UDP + chrysin 7-O-beta-D-glucoside
-
?
UDP-glucose + eriodictyol 48% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + eriodictyol 7-O-beta-D-glucoside
-
?
UDP-glucose + esculetin 77% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + esculetin 6-O-beta-D-glucoside
-
?
UDP-glucose + kaempferol-4'-methylether 42% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + kaempferol-4'-methylether 7-O-beta-D-glucoside
-
?
UDP-glucose + luteolin 17% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + luteolin 7-O-beta-D-glucoside
-
?
UDP-glucose + luteolin as active as with quercetin, UGT73A4 Beta vulgaris UDP + luteolin 7-O-beta-D-glucoside
-
?
UDP-glucose + myricetin 80% of the activity with quercetin, 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 Beta vulgaris UDP + myricetin 4'-O-beta-D-glucoside
-
?
UDP-glucose + naringenin 10% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + naringenin 7-O-beta-D-glucoside
-
?
UDP-glucose + pinocembrin 25% of the activity with quercetinUGT73A4 Beta vulgaris UDP + pinocembrin 7-O-beta-D-glucoside
-
?
UDP-glucose + pinocembrin 57% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + pinocembrin 7-O-beta-D-glucoside
-
?
UDP-glucose + quercetagetin 84% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + quercetagetin 3-O-beta-D-glucoside + quercetagetin 7-O-beta-D-glucoside
-
?
UDP-glucose + quercetagetin 28% of the activity with quercetin, 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 Beta vulgaris UDP + quercetagetin 4'-O-beta-D-glucoside
-
?

Subunits

Subunits Comment Organism
monomer 1 * 54070, calculated from sequence, UGT73A4 Beta vulgaris
monomer 1 * 54390, calculated from sequence, UGT71F1 Beta vulgaris

Synonyms

Synonyms Comment Organism
UGT71F1
-
Beta vulgaris
UGT73A4
-
Beta vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
UGT73A4 Beta vulgaris
37
-
UGT71F1 Beta vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
UGT73A4 Beta vulgaris
7 9 UGT71F1 Beta vulgaris

pI Value

Organism Comment pI Value Maximum pI Value
Beta vulgaris calculated from sequence, MALDI-TOF-MS analysis with molecular masses of tags subtracted, UGT71F1
-
5.6
Beta vulgaris calculated from sequence, MALDI-TOF-MS analysis with molecular masses of tags subtracted, UGT73A4
-
5.8