Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.237 extracted from

  • Taguchi, G.; Ubukata, T.; Hayashida, N.; Yamamoto, H.; Okazaki, M.
    Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxylgroup of coumarin from tobacco cells (2003), Arch. Biochem. Biophys., 420, 95-102.
    View publication on PubMed
Search for more literature for 2.4.1.237

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme expression is inducible by plant hormones, such as salicylic acid, auxin, 2,4-dichorophenoxyacetic acid, and jasmonate Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
 kaempferol pH 8.0, 30°C, recombinant enzyme Nicotiana tabacum
0.0236
-
 3-hydroxycoumarin pH 8.0, 30°C, recombinant enzyme Nicotiana tabacum
0.0432
-
 UDP-glucose pH 8.0, 30°C, recombinant enzyme Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Nicotiana tabacum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information broad substrate specificity, no activity with salicylic acid, cyanidin, and cyanidin 3-O-glucoside, poor activity with 4-hydroxycoumarin and 5-hydroxyflavone Nicotiana tabacum ?
-
 ?
UDP-alpha-D-glucose + kaempferol preferred substrate Nicotiana tabacum UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + quercetin 19% of the activity with kaempferol Nicotiana tabacum UDP + quercetin 7-O-beta-D-glucoside
-
 ?
UDP-glucose + 1-naphthol 44% of the activity with kaempferol Nicotiana tabacum UDP + naphthol 1-O-beta-D-glucoside
-
 ?
UDP-glucose + 2-coumaric acid 41% of the activity with kaempferol Nicotiana tabacum UDP + ?
-
 ?
UDP-glucose + 2-naphthol 14% of the activity with kaempferol Nicotiana tabacum UDP + naphthol 2-O-beta-D-glucoside
-
 ?
UDP-glucose + 3-hydroxycoumarin 310% of the activity with kaempferol Nicotiana tabacum UDP + coumarin 3-O-beta-D-glucoside
-
 ?
UDP-glucose + 3-hydroxyflavone 12% of the activity with kaempferol Nicotiana tabacum UDP + 3-hydroxyflavone 7-O-beta-D-glucoside
-
 ?
UDP-glucose + 4-coumaric acid 43% of the activity with kaempferol Nicotiana tabacum UDP + ?
-
 ?
UDP-glucose + 6-hydroxyflavone 24% of the activity with kaempferol Nicotiana tabacum UDP + 6-hydroxyflavone 7-O-beta-D-glucoside
-
 ?
UDP-glucose + 7-hydroxyflavone 53% of the activity with kaempferol Nicotiana tabacum UDP + 7-hydroxyflavone 7-O-beta-D-glucoside
-
 ?
UDP-glucose + esculetin 24% of the activity with kaempferol Nicotiana tabacum UDP + esculetin 7-O-beta-D-glucoside
-
 ?
UDP-glucose + kaempferol 3-O-glucoside 45% of the activity with kaempferol Nicotiana tabacum UDP + kaempferol 3,7beta-O-diglucoside
-
 ?
UDP-glucose + scopoletin 11% of the activity with kaempferol Nicotiana tabacum UDP + scopoletin 7-O-beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
GT2
-
 Nicotiana tabacum
NTGT2
-
 Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 with kaempferol as substrate Nicotiana tabacum