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Literature summary for 2.4.1.236 extracted from

  • Frydman, A.; Liberman, R.; Huhman, D.V.; Carmeli-Weissberg, M.; Sapir-Mir, M.; Ophir, R.; W Sumner, L.; Eyal, Y.
    The molecular and enzymatic basis of bitter/non-bitter flavor of citrus fruit: evolution of branch-forming rhamnosyltransferases under domestication (2013), Plant J., 73, 166-178.
    View publication on PubMed
Search for more literature for 2.4.1.236

Cloned(Commentary)

Cloned (Comment) Organism
1,2RhaT cloning and DNA and amino acid sequence determination and analysis, phylogenetic analysis, two native frameshift mutations render the enzyme from orange catalytically inactive Citrus sinensis
phylogenetic analysis, 1,2RhaT expressionin BY-2 cells Citrus maxima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Citrus maxima the enzyyme specifically catalyzes rhamnosylation of flavanone-7-O-glucose and flavone-7-O-glucose substrates at position 2 of the glucose moiety ?
-
 ?
UDP-L-rhamnose + diosmetin 7-O-glucoside Citrus maxima a flavone 7-O-glucoside UDP + diosmetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neodiosmin, a bitter 7-O-neohesperidoside ?
UDP-L-rhamnose + hesperetin 7-O-glucoside Citrus maxima
-
 UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neohesperidin, a bitter 7-O-neohesperidoside ?
UDP-L-rhamnose + naringenin 7-O-glucoside Citrus maxima
-
 UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. naringin, a bitter 7-O-neohesperidoside ?

Organism

Organism UniProt Comment Textmining
Citrus maxima
-
-
-
Citrus sinensis
-
-
-
Citrus sinensis valencia
-
-
-
no activity in Citrus reticulata
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Citrus maxima
-
leaf
-
 Citrus maxima
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyyme specifically catalyzes rhamnosylation of flavanone-7-O-glucose and flavone-7-O-glucose substrates at position 2 of the glucose moiety Citrus maxima ?
-
 ?
additional information the enzyme also catalyzes the rhamnosylation of diosmetin 7-O-glucoside at position 2 of the glucose moiety to give neodiosmin, but Cm1,2RhaT is specific for flavonoid-7-O-glucosides and utilizes no flavonoid-3-O-glucosides Citrus maxima ?
-
 ?
UDP-L-rhamnose + diosmetin 7-O-glucoside a flavone 7-O-glucoside Citrus maxima UDP + diosmetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neodiosmin, a bitter 7-O-neohesperidoside ?
UDP-L-rhamnose + hesperetin 7-O-glucoside
-
 Citrus maxima UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neohesperidin, a bitter 7-O-neohesperidoside ?
UDP-L-rhamnose + hesperetin 7-O-glucoside a flavanone 7-O-glucoside Citrus maxima UDP + hesperetin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. neohesperidin, a bitter 7-O-neohesperidoside ?
UDP-L-rhamnose + naringenin 7-O-glucoside
-
 Citrus maxima UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. naringin, a bitter 7-O-neohesperidoside ?
UDP-L-rhamnose + naringenin 7-O-glucoside a flavanone 7-O-glucoside Citrus maxima UDP + naringenin 7-O-[beta-L-rhamnosyl-(1-2)-beta-D-glucoside] i.e. naringin, a bitter 7-O-neohesperidoside ?

Synonyms

Synonyms Comment Organism
1,2-rhamnosyltransferase
-
 Citrus sinensis
1,2-rhamnosyltransferase
-
 Citrus maxima
1,2RhaT
-
 Citrus sinensis
1,2RhaT
-
 Citrus maxima
flavanone-7-O-glucose-1,2-rhamnosyltransferase
-
 Citrus sinensis
flavanone-7-O-glucose-1,2-rhamnosyltransferase
-
 Citrus maxima

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of flavonoid branched-chain glycosides in citrus, overview. 1,2RhaT is the key branch-forming enzyme directing biosynthesis of the bitter flavanone neohesperidoside compounds in bitter Citrus species Citrus maxima