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Literature summary for 2.4.1.231 extracted from
- Characterization of trehalose phosphorylase from Schizophyllum commune (1999), Biochem. J., 341, 385-393.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | competitive with respect to phosphate | Schizophyllum commune |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
phosphate | 30°C, pH 6.6 | Schizophyllum commune | |
| 2.8 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 6.6 | Schizophyllum commune | |
| 39 | - |
alpha-D-glucose | 30°C, pH 6.6 | Schizophyllum commune | |
| 91 | - |
alpha,alpha-trehalose | 30°C, pH 6.6 | Schizophyllum commune | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Schizophyllum commune |
| 61000 | - |
SDS-PAGE | Schizophyllum commune |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + phosphate | Schizophyllum commune | - |
alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizophyllum commune | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Schizophyllum commune |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate | mechanism | Schizophyllum commune |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.85 | - |
- |
Schizophyllum commune |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + arsenate | - |
Schizophyllum commune | alpha-D-glucose 1-arsenate + alpha-D-glucose | - |
r | |
| alpha,alpha-trehalose + phosphate | - |
Schizophyllum commune | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE, gel filtration | Schizophyllum commune |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
unstable, loses activity within a few hours, glycerol, PEG 4000 or trehalose stabilize | Schizophyllum commune |
| 20 | - |
unstable, loses activity within a few hours, glycerol, PEG 4000 or trehalose stabilize | Schizophyllum commune |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 6.6 | Schizophyllum commune | |
| 10 | - |
alpha-D-glucose | 30°C, pH 6.6 | Schizophyllum commune | |
| 14 | - |
phosphate | 30°C, pH 6.6 | Schizophyllum commune | |
| 15 | - |
alpha,alpha-trehalose | 30°C, pH 6.6 | Schizophyllum commune | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
reverse reaction | Schizophyllum commune |
| 6.6 | - |
forward reaction | Schizophyllum commune |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 6.6 | decrease in enzyme activity below pH 6.2 and above pH 6.6 | Schizophyllum commune |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 67 | - |
NaCl | 30°C, pH 6.6 | Schizophyllum commune | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Schizophyllum commune | isoelectric focusing between pH 4 and pH 6.5 | - |
4.8 |
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