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Literature summary for 2.4.1.230 extracted from

  • Yamamoto, T.; Maruta, K.; Mukai, K.; Yamashita, H.; Nishimoto, T.; Kubota, M.; Fukuda, S.; Kurimoto, M.; Tsujisaka, Y.
    Cloning and sequencing of kojibiose phosphorylase gene from Thermoanaerobacter brockii ATCC35047 (2004), J. Biosci. Bioeng., 98, 99-106.
    View publication on PubMed
Search for more literature for 2.4.1.230

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermoanaerobacter brockii

Protein Variants

Protein Variants Comment Organism
D177N 106% of wild-type activity Thermoanaerobacter brockii
D271N 69% of wild-type activity Thermoanaerobacter brockii
D340N 89% of wild-type activity Thermoanaerobacter brockii
D362N complete loss of enzymic activity Thermoanaerobacter brockii
D459N increase in Km-values for kojibiose, beta-D-glucose 1-phosphate and glucose Thermoanaerobacter brockii
E284Q 103% of wild-type activity Thermoanaerobacter brockii
E642Q complete loss of enzymic activity Thermoanaerobacter brockii
K114Q 85% of wild-type activity Thermoanaerobacter brockii
K403Q 73% of wild-type activity Thermoanaerobacter brockii
K614Q complete loss of enzymic activity Thermoanaerobacter brockii
K749Q 78% of wild-type activity Thermoanaerobacter brockii
R137Q 61% of wild-type activity Thermoanaerobacter brockii
R33Q 31% of wild-type activity Thermoanaerobacter brockii
R476Q 50% of wild-type activity Thermoanaerobacter brockii
R48Q 62% of wild-type activity Thermoanaerobacter brockii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 phosphate wild-type, pH 5.5, 60°C Thermoanaerobacter brockii
0.79
-
 beta-D-glucose 1-phosphate wild-type, pH 5.5, 60°C Thermoanaerobacter brockii
0.8
-
 kojibiose wild-type, pH 5.5, 60°C Thermoanaerobacter brockii
1.11
-
 phosphate mutant D459N, pH 5.5, 60°C Thermoanaerobacter brockii
3.84
-
 beta-D-glucose wild-type, pH 5.5, 60°C Thermoanaerobacter brockii
5.21
-
 kojibiose mutant D459N, pH 5.5, 60°C Thermoanaerobacter brockii
8.57
-
 beta-D-glucose 1-phosphate mutant D459N, pH 5.5, 60°C Thermoanaerobacter brockii
25.4
-
 beta-D-glucose mutant D459N, pH 5.5, 60°C Thermoanaerobacter brockii

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter brockii
-
 strain ATCC35047, expression in Escherichia coli
-
Thermoanaerobacter brockii ATCC 35047
-
 strain ATCC35047, expression in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + beta-D-glucose 1-phosphate
-
 Thermoanaerobacter brockii 2-alpha-D-glucosyl-D-glucose + phosphate
-
 r
beta-D-glucose + beta-D-glucose 1-phosphate
-
 Thermoanaerobacter brockii ATCC 35047 2-alpha-D-glucosyl-D-glucose + phosphate
-
 r
kojibiose + phosphate
-
 Thermoanaerobacter brockii beta-D-glucose-1-phosphate + D-glucose
-
 r
kojibiose + phosphate
-
 Thermoanaerobacter brockii ATCC 35047 beta-D-glucose-1-phosphate + D-glucose
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermoanaerobacter brockii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Thermoanaerobacter brockii