BRENDA - Enzyme Database
show all sequences of 2.4.1.230

Enzymatic synthesis of a 2-O-alpha-D-glucopyranosyl cyclic tetrasaccharide by kojibiose phosphorylase

Watanabe, H.; Higashiyama, T.; Aga, H.; Nishimoto, T.; Kubota, M.; Fukuda, S.; Kurimoto, M.; Tsujisaka, Y.; Carbohydr. Res. 340, 449-454 (2005) View publication on PubMed

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Thermoanaerobacter brockii
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cyclo-[(-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-)] + beta-D-glucose 1-phosphate
i.e. CTS
658401
Thermoanaerobacter brockii
cyclo-[(-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-[alpha-D-Glcp-(1-2)]-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-)] + phosphate
major product, i.e. 2-O-alpha-D-glucopyranosyl-CTS
-
-
?
additional information
enzyme also catalyzes the chain-extending reaction of the side chain of 2-O-alpha-D-glucopyranosyl-CTS
658401
Thermoanaerobacter brockii
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Thermoanaerobacter brockii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
assay at
Thermoanaerobacter brockii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cyclo-[(-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-)] + beta-D-glucose 1-phosphate
i.e. CTS
658401
Thermoanaerobacter brockii
cyclo-[(-6)-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-6)-[alpha-D-Glcp-(1-2)]-alpha-D-Glcp-(1-3)-alpha-D-Glcp-(1-)] + phosphate
major product, i.e. 2-O-alpha-D-glucopyranosyl-CTS
-
-
?
additional information
enzyme also catalyzes the chain-extending reaction of the side chain of 2-O-alpha-D-glucopyranosyl-CTS
658401
Thermoanaerobacter brockii
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Thermoanaerobacter brockii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
assay at
Thermoanaerobacter brockii
Other publictions for EC 2.4.1.230
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
758811
Mukherjee
Discovery of a kojibiose phos ...
Escherichia coli, Escherichia coli MG1655
Biochemistry
57
2857-2867
2018
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1
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1
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5
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10
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1
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1
1
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1
1
725294
Jung
Identification and characteriz ...
Pyrococcus sp., Pyrococcus sp. ST04
J. Bacteriol.
196
1122-1131
2014
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1
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2
3
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4
1
2
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8
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8
1
1
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3
2
2
1
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1
1
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2
2
736088
Okada
Structural and mutational anal ...
Caldicellulosiruptor saccharolyticus
FEBS J.
281
778-786
2014
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1
1
1
9
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1
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3
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5
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1
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1
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2
2
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721869
Yamamoto
Enzymatic properties of recomb ...
Caldicellulosiruptor saccharolyticus, Caldicellulosiruptor saccharolyticus ATCC 43494
Biosci. Biotechnol. Biochem.
75
1208-1210
2011
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1
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2
2
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7
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2
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8
1
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2
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2
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2
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2
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8
1
2
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2
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2
-
2
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1
1
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720962
Nakai
Rational engineering of Lactob ...
Thermoanaerobacter brockii, Lactobacillus acidophilus, Thermoanaerobacter brockii ATCC 35047
Protein Eng. Des. Sel.
23
781-787
2010
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1
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1
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3
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2
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8
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1
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1
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2
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1
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4
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1
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3
2
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4
4
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3
3
691751
Takahashi
Three novel oligosaccharides s ...
Thermoanaerobacter brockii
Chem. Cent. J.
1
18
2007
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1
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4
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1
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4
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692803
Takahashi
-
Isolation and identification o ...
Thermoanaerobacter brockii
J. Appl. Glycosci.
54
195-200
2007
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1
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1
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1
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1
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5
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672930
Yamamoto
Construction and characterizat ...
Thermoanaerobacter brockii
Carbohydr. Res.
341
2350-2359
2006
-
-
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1
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6
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1
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1
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1
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1
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674952
Yamamoto
Acceptor recognition of kojibi ...
Thermoanaerobacter brockii
J. Biosci. Bioeng.
101
427-433
2006
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4
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4
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4
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658401
Watanabe
Enzymatic synthesis of a 2-O-a ...
Thermoanaerobacter brockii
Carbohydr. Res.
340
449-454
2005
-
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3
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2
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1
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1
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659532
Yamamoto
Enhancement of thermostability ...
Thermoanaerobacter brockii
J. Biosci. Bioeng.
100
212-215
2005
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1
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6
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1
1
3
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1
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660528
Takahashi
-
Structural analysis of six nov ...
Thermoanaerobacter brockii
Tetrahedron
16
57-63
2005
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2
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1
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1
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674949
Yamamoto
Hyper expression of kojibiose ...
Thermoanaerobacter brockii, Thermoanaerobacter brockii TCC35047
J. Biosci. Bioeng.
100
343-346
2005
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1
1
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10
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659537
Yamamoto
Cloning and sequencing of koji ...
Thermoanaerobacter brockii, Thermoanaerobacter brockii ATCC 35047
J. Biosci. Bioeng.
98
99-106
2004
-
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1
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15
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8
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11
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4
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1
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1
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1
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15
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4
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1
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1
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644896
Okada
Synthesis and structural analy ...
Thermoanaerobacter brockii
Carbohydr. Res.
338
879-885
2003
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4
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644895
Chaen
Enzymatic synthesis of kojioli ...
Thermoanaerobacter brockii
J. Biosci. Bioeng.
92
177-182
2001
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644894
Chaen
-
Purification and characterizat ...
Thermoanaerobacter brockii
J. Appl. Glycosci.
46
423-429
1999
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4
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