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Literature summary for 2.4.1.23 extracted from

  • Cleland, W.W.; Kennedy, E.P.
    The enzymatic synthesis of psychosine (1960), J. Biol. Chem., 235, 45-51.
    View publication on PubMed
Search for more literature for 2.4.1.23

Activating Compound

Activating Compound Comment Organism Structure
Tween-20 4 mg/ml, highest activity with DL-erythro-trans-sphingosine, solubilizing effect Cavia porcellus
Tween-20 4 mg/ml, highest activity with DL-erythro-trans-sphingosine, solubilizing effect Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 UDPgalactose
-
 Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Cavia porcellus
-
-
microsome
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2-8 mM, stimulation Cavia porcellus
Mn2+ 2-3 mM, slight stimulation Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Cavia porcellus
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Cavia porcellus
-
brain
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
lyophilized powder is indefinitely stable in the deep-frozen Cavia porcellus
lyophilized powder is indefinitely stable in the deep-frozen Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPgalactose + D-1,3,4-trihydroxy-2-aminooctadecane weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-1-hydroxy-2-amino-octadecane
-
 Rattus norvegicus ?
-
 ?
UDPgalactose + DL-1-hydroxy-2-amino-octadecane weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-erythro-1,3-dihydroxy-2-amino-4-cis-octadecene
-
 Rattus norvegicus ?
-
 ?
UDPgalactose + DL-erythro-1,3-dihydroxy-2-amino-4-cis-octadecene weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-erythro-1,3-dihydroxy-2-amino-4-heptadecyne weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-erythro-trans-sphingosine maximal activity Rattus norvegicus UDP + psychosine
-
 ?
UDPgalactose + DL-erythro-trans-sphingosine maximal activity Cavia porcellus UDP + psychosine i. e. galactosylsphingosine ?
UDPgalactose + DL-erythro-trans-sphingosine basic structure for the acceptor seems to be a 1-hydroxy-2-amino hydrocarbon of about 18 carbon atoms Rattus norvegicus UDP + psychosine
-
 ?
UDPgalactose + DL-erythro-trans-sphingosine basic structure for the acceptor seems to be a 1-hydroxy-2-amino hydrocarbon of about 18 carbon atoms Cavia porcellus UDP + psychosine i. e. galactosylsphingosine ?
UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-cis-octadecene weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-octadecyne
-
 Rattus norvegicus ?
-
 ?
UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-octadecyne weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-trans-heptadecene weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-trans-nonadecene weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-trans-octadecene weak activity Cavia porcellus ?
-
 ?
UDPgalactose + DL-threo-1,3-dihydroxy-2-aminooctadecane weak activity Cavia porcellus ?
-
 ?
UDPgalactose + N-acetyl-DL-threo-trans-sphingosine
-
 Cavia porcellus UDP + N-acetylpsychosine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cavia porcellus
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Cavia porcellus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 70% of maximal activity at pH 7, 85% of maximal activity at pH 9.0 Cavia porcellus