Cloned (Comment) | Organism |
---|---|
cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein | Dictyostelium discoideum |
cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein | Dictyostelium purpureum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | Dictyostelium discoideum | - |
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | Dictyostelium purpureum | - |
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Dictyostelium purpureum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged hybrid Gnt1, formed of Dictyostelium discoideum Gnt1/Dictyostelium purpureum Gnt1, from Escherichia coli by nickel affinity chromatography | Dictyostelium purpureum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity of recombinant Gnt1 hydrid enzyme, although synthetic 13-mer and 23-mer hydroxyproline peptides are substrates than Skyp1A, their activities are much lower even at 5000fold higher concentration, the 40-mer hydroxyproline peptide is also a poor substrate, overview | Dictyostelium purpureum | ? | - |
? | |
additional information | substrate specificity of recombinant Gnt1 hydrid enzyme, although synthetic 13-mer and 23-mer hydroxyproline peptides are substrates, their activities are much lower even at 5000fold higher concentration, the 40-mer hydroxyproline peptide is also a poor substrate, overview | Dictyostelium discoideum | ? | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | - |
Dictyostelium discoideum | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | - |
Dictyostelium purpureum | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | transfer to the hydroxyproline, at the Thr-Pro143 dipeptide, preceded by a coil and succeeded by an alpha-helix in Skp1 | Dictyostelium discoideum | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | transfer to the hydroxyproline, at the Thr-Pro143 dipeptide, preceded by a coil and succeeded by an alpha-helix in Skp1 | Dictyostelium purpureum | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GnT1 | - |
Dictyostelium discoideum |
GnT1 | - |
Dictyostelium purpureum |
hydroxyproline-capping enzyme | - |
Dictyostelium discoideum |
hydroxyproline-capping enzyme | - |
Dictyostelium purpureum |
General Information | Comment | Organism |
---|---|---|
metabolism | a hypoxia inducible factor-alpha (HIFR) type prolyl 4-hydroxylase, P4H1, and an alpha-N-acetylglucosaminyltransferase Gnt1, sequentially modify proline 143 of Skp1, a subunit of the Skp1/Cullin/F-box protein, SCF, class of E3 ubiquitin ligases | Dictyostelium discoideum |
metabolism | a hypoxia inducible factor-alpha (HIFR) type prolyl 4-hydroxylase, P4H1, and an alpha-N-acetylglucosaminyltransferase Gnt1, sequentially modify proline 143 of Skp1, a subunit of the Skp1/Cullin/F-box protein, SCF, class of E3 ubiquitin ligases | Dictyostelium purpureum |
additional information | Gnt1 from Dictyostelium discoideum contains a stretch of 80 Asn residues and other Asn-rich inserts | Dictyostelium discoideum |
additional information | Gnt1 from Dictyostelium purureum lacks a stretch of 80 Asn residues and other Asn-rich inserts | Dictyostelium purpureum |