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Literature summary for 2.4.1.229 extracted from

  • Teng-umnuay, P.; van der Wel, H.; West, C.M.
    Identification of a UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase in the cytoplasm of Dictyostelium (1999), J. Biol. Chem., 274, 36392-36402.
    View publication on PubMed
Search for more literature for 2.4.1.229

Activating Compound

Activating Compound Comment Organism Structure
Brij-35 slightly stimulating Dictyostelium sp.
dithiothreitol activates, best at 5 mM Dictyostelium sp.
additional information requires reducing condition Dictyostelium sp.
NP-40 slightly stimulating Dictyostelium sp.
Triton X-100 slightly stimulating Dictyostelium sp.
Tween 20 stimulates, less efficient than Tween 80 Dictyostelium sp.
Tween 80 stimulates Dictyostelium sp.
Uracil stimulates the purified enzyme at 0.1 mM Dictyostelium sp.

General Stability

General Stability Organism
Tween 20 and Tween 80 stabilize Dictyostelium sp.

Inhibitors

Inhibitors Comment Organism Structure
ATP weak inhibition Dictyostelium sp.
Ca2+
-
 Dictyostelium sp.
CTP weak inhibition Dictyostelium sp.
EDTA complete inhibition at 5 mM Dictyostelium sp.
K+ weak Dictyostelium sp.
Mn2+
-
 Dictyostelium sp.
additional information Skp-1-protein isoform containing unhydroxylated proline143 is probably inhibitory Dictyostelium sp.
Na+ inhibitory at concentrations above 0.05 M Dictyostelium sp.
UDP
-
 Dictyostelium sp.
UMP best inhibitor Dictyostelium sp.
UTP
-
 Dictyostelium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00016
-
 UDP-GlcNAc pH 7.8, 30°C Dictyostelium sp.
0.00056
-
 [Skp1-protein]-trans-4-hydroxy-L-proline pH 7.8, 30°C Dictyostelium sp.
1.6
-
 hydroxyproline-peptide pH 7.8, 30°C Dictyostelium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Dictyostelium sp. 5829
-
additional information no activity in the particulate fraction Dictyostelium sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates, best at 10 mM Dictyostelium sp.
additional information divalent cations required Dictyostelium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Dictyostelium sp.
51000
-
 1 * 51000, SDS-PAGE Dictyostelium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline Dictyostelium sp.
-
 UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
130000fold Dictyostelium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00023
-
 purified enzyme Dictyostelium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + [Skp1-protein]-trans-4-hydroxy-L-proline
-
 Dictyostelium sp. UDP + [Skp1-protein]-O-(N-acetyl-alpha-D-glucosaminyl)-trans-4-hydroxy-L-proline
-
 ?
UDP-N-acetylglucosamine + hydroxyproline-peptide synthetic peptide substrate Dictyostelium sp. UDP + (N-acetyl-D-glucosaminyl)hydroxyproline peptide
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline hydroxyproline143 Dictyostelium sp. UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline high affinity for UDP-GlcNAc Dictyostelium sp. UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline recombinant protein substrate, deficient in GlcNAc Dictyostelium sp. UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline
-
 Dictyostelium sp. UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 51000, SDS-PAGE Dictyostelium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Dictyostelium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 partially purified enzyme, unstable Dictyostelium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Dictyostelium sp.