Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.228 extracted from

  • Kaczmarek, R.; Duk, M.; Szymczak, K.; Korchagina, E.; Tyborowska, J.; Mikolajczyk, K.; Bovin, N.; Szewczyk, B.; Jaskiewicz, E.; Czerwinski, M.
    Human Gb3/CD77 synthase reveals specificity toward two or four different acceptors depending on amino acid at position 211, creating Pk, P1 and NOR blood group antigens (2016), Biochem. Biophys. Res. Commun., 470, 168-174.
    View publication on PubMed
Search for more literature for 2.4.1.228

Cloned(Commentary)

Cloned (Comment) Organism
gene A4GALT, a fragment of the A4GALT gene spanning nucleotides 130-1059 of the open reading frame with C or G at position 631 respectively, is cloned in-frame with c-myc and hexahistidine tags and recombinantly expressed in Spodoptera frugiperda Sf9 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
Q211E a mutation in the open reading frame, which broadens the enzyme specificity, making it able also to synthesize Gal(alpha1-4)GalNAc moiety, which constitutes the defining terminal disaccharide of the NOR antigen (carried by two glycosphingolipids: NOR1 and NOR2) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
 x * 37500, recombinant c-Myc- and His6-tagged fragment A4GALT(130-1059), SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NPC4 gene A4GALT
-

Purification (Commentary)

Purification (Comment) Organism
recombinant c-Myc- and His6-tagged fragment A4GALT(130-1059) from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the consensus enzyme synthesizes both the Pk and P1 antigens, while its p.Q211E variant additionally synthesizes the NOR antigen Homo sapiens ?
-
 ?
UDP-Gal + Galbeta1,4Glc-PAA i.e. Lac-PAA, precursor to Pk Homo sapiens UDP + ?
-
 ?
UDP-Gal + Galbeta1,4GlcNAcbeta1,3Galbeta1,4Glc-PAA i.e. nLc4-PAA, precursor to P1 Homo sapiens UDP + ?
-
 ?
UDP-Gal + GalNAcbeta1,3Galalpha1,4Galbeta1,4Glc-PAA i.e. Gbeta4-PAA, precursor to NOR1 Homo sapiens UDP + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37500, recombinant c-Myc- and His6-tagged fragment A4GALT(130-1059), SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha1,4-galactosyltransferase
-
 Homo sapiens
Gb3/CD77 synthase
-
 Homo sapiens
Pk synthase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
 about, assay at Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the synthesis of Gal(alpha1-4)Gal moiety in globotriaosylceramide (Gb3Cer, CD77, Pk blood group antigen), a glycosphingolipid of the globo series Homo sapiens