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Literature summary for 2.4.1.226 extracted from

  • Hu, B.; Shi, C.; Tian, Y.; Zhang, Y.; Xu, C.; Chen, H.; Cao, P.; Yuan, W.
    TGF-beta induces up-regulation of chondroitin sulfate synthase 1 (CHSY1) in nucleus pulposus cells through MAPK signaling (2015), Cell. Physiol. Biochem., 37, 793-804.
    View publication on PubMed
Search for more literature for 2.4.1.226

Cloned(Commentary)

Cloned (Comment) Organism
gene chsy1, real-time PCR enzyme expression analysis Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information gene silencing via shRNA sequences targeting Rattus norvegicus Smad2, Smad3, CHSY1, c-jun and Sp1, the shRNAs are transfected using a lentiviral vector Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan Rattus norvegicus
-
 UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 gene CHSY1
-

Source Tissue

Source Tissue Comment Organism Textmining
nucleus pulposus
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
-
 Rattus norvegicus UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
-
 ?

Synonyms

Synonyms Comment Organism
chondroitin sulfate synthase 1
-
 Rattus norvegicus
CHSY1
-
 Rattus norvegicus

Expression

Organism Comment Expression
Rattus norvegicus knockdown of c-Jun and Sp1 decreases CHSY1 promoter activity, CHSY1 expression and sulfated glycosaminoglycan accumulation induced by TGF-beta down
Rattus norvegicus TGF-beta induces up-regulation of CHSY1 in nucleus pulposus cells through MAPK signaling. TGF-beta increases CHSY1 expression and promoter activity in the nucleus pulposus partially through activation of canonical Smad signaling pathway up

General Information

General Information Comment Organism
malfunction silencing of CHSY1 decreases sulfated glycosaminoglycan accumulation in nucleus pulposus cells induced by TGF-beta Rattus norvegicus
physiological function chondroitin sulfate synthase 1 (CHSY1) is a glycosyltransferases involved in the biosynthesis of chondroitin and dermatan sulfate glycosaminoglycan. CHSY1 possesses dual glucuronyl-transferase and galactosaminyl-transferase activity. It can promote the transfer of both GlcUA and GalNAc from their donors UDP-GlcUA and UDP-GalNAc, respectively, to the non-reducing end of the chondroitin polymer, resulting in the elongating and accumulation of glycosaminoglycans Rattus norvegicus