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Literature summary for 2.4.1.226 extracted from

  • Kitagawa, H.; Ujikawa, M.; Tsutsumi, K.; Tamura, J.I.; Neumann, K.W.; Ogawa, T.; Sugahara, K.
    Characterization of serum beta-glucuronyltransferase involved in chondroitin sulfate biosynthesis (1997), Glycobiology, 7, 905-911.
    View publication on PubMed
Search for more literature for 2.4.1.226

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.051
-
 UDP-glucuronic acid
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan Bos taurus
-
 UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Bos taurus

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine transfer to the acceptor through a beta-linkage Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
blood serum fetal bovine serum Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information further substrates: chondroitin heptaserine, pentaserine, non-sulfated odd-numbered oligosaccharides with a N-acetylgalactosamine residue at the non-reducing terminus except for a trisaccharide Bos taurus ?
-
 ?
additional information no substrates: a trisaccharide-serine, an alpha-N-acetylgalactosamine-capped pentasaccharide-serine Bos taurus ?
-
 ?
additional information the glucuronic acid transfer rate roughly increases with increasing chain length Bos taurus ?
-
 ?
additional information 6-O-sulfation of non-reducing terminal N-acetylgalactosamine markedly enhances glucuronic acid transfer Bos taurus ?
-
 ?
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 Bos taurus UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
-
 ?
UDP-glucuronic acid + chondroitin
-
 Bos taurus UDP + N-acetylchondrosine glucuronic acid beta(1-3)N-acetylgalactosamine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 sodium acetate buffer Bos taurus