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Literature summary for 2.4.1.224 extracted from
- Determination of the substrate specificities of N-acetyl-D-glucosaminyltransferase (2006), Biochemistry, 45, 12358-12365.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in a C-terminal six-His fusion protein in BL21 star cells coexpressing chaperone proteins GroEL and GroES | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM-values for the disaccahride, tetrasaccharide, hexasaccharide and octasaccharide acceptors | Escherichia coli | |
| 0.094 | - |
UDP-N-acetyl-D-glucosamine | pH 7.2 | Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1,4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan | - |
Escherichia coli | UDP + N-acetyl-alpha-D-glucosaminyl-(1,4)-beta-D-glucuronosyl-(1,4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KfiA | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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