BRENDA - Enzyme Database
show all sequences of 2.4.1.223

Human tumor suppressor EXT gene family members EXTL1 and EXTL3 encode alpha-1,4-N-acetylglucosaminyltransferases that likely are involved in heparan sulfate/heparin biosynthesis

Kim, B.T.; Kitagawa, H.; Tamura, J.I.; Saito, T.; Kusche-Gullberg, M.; Lindahl, U.; Sugahara, K.; Proc. Natl. Acad. Sci. USA 98, 7176-7181 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in COS-1 cells, lacking the putative NH2-terminal transmembrane and cytoplasmic domains
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
Homo sapiens
essential in the heparan sulfate biosynthesis
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
genes EXTL1 and EXTL3
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
488960
Homo sapiens
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
essential in the heparan sulfate biosynthesis
488960
Homo sapiens
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
-
488960
Homo sapiens
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in COS-1 cells, lacking the putative NH2-terminal transmembrane and cytoplasmic domains
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
Homo sapiens
essential in the heparan sulfate biosynthesis
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
488960
Homo sapiens
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
essential in the heparan sulfate biosynthesis
488960
Homo sapiens
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
-
488960
Homo sapiens
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
-
-
-
?
Other publictions for EC 2.4.1.223
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
686299
Herr
Regulation of TGF-beta signall ...
Xenopus laevis
Development
135
1813-1822
2008
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661562
Han
Distinct and collaborative rol ...
Drosophila melanogaster
Development
131
1563-1575
2005
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662163
Kim
In vitro heparan sulfate polym ...
Homo sapiens
J. Biol. Chem.
278
41618-41623
2003
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1
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488961
Kim
Demonstration of a novel gene ...
Drosophila melanogaster
J. Biol. Chem.
277
13659-13665
2002
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1
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488951
Kitagawa
rib-2, a Caenorhabditis elegan ...
Caenorhabditis elegans
J. Biol. Chem.
276
4834-4838
2001
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1
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488960
Kim
Human tumor suppressor EXT gen ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
98
7176-7181
2001
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1
-
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-
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1
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1
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1
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662102
Kitagawa
The tumor suppressor EXT-like ...
Homo sapiens
J. Biol. Chem.
274
13933-13937
1999
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