BRENDA - Enzyme Database
show all sequences of 2.4.1.223

rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha-1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate

Kitagawa, H.; Egusa, N.; Tamura, J.I.; Kusche-Gullberg, M.; Lindahl, U.; Sugahara, K.; J. Biol. Chem. 276, 4834-4838 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in COS-1 cells, truncated form , lacking the first 58 N-terminal amino acids
Caenorhabditis elegans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
Caenorhabditis elegans
initiation and elongation of heparan sulfate
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caenorhabditis elegans
-
gene rib-2
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
initiation and elongation of heparan sulfate
488951
Caenorhabditis elegans
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
-
488951
Caenorhabditis elegans
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in COS-1 cells, truncated form , lacking the first 58 N-terminal amino acids
Caenorhabditis elegans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
Caenorhabditis elegans
initiation and elongation of heparan sulfate
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
initiation and elongation of heparan sulfate
488951
Caenorhabditis elegans
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-xylosyl-proteoglycan
-
-
-
-
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
-
488951
Caenorhabditis elegans
UDP + alpha-N-acetyl-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-beta-D-galactosyl-1-O-benzyloxycarbonyl
-
-
-
?
Other publictions for EC 2.4.1.223
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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662163
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Homo sapiens
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488961
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Demonstration of a novel gene ...
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488951
Kitagawa
rib-2, a Caenorhabditis elegan ...
Caenorhabditis elegans
J. Biol. Chem.
276
4834-4838
2001
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1
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1
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3
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488960
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Human tumor suppressor EXT gen ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
98
7176-7181
2001
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1
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1
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1
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662102
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Homo sapiens
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274
13933-13937
1999
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