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Literature summary for 2.4.1.222 extracted from

  • Hou, X.; Tashima, Y.; Stanley, P.
    Galactose differentially modulates Lunatic and Manic Fringe effects on Delta1-induced NOTCH signaling (2012), J. Biol. Chem., 287, 474-483.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
D-galactose Gal is required for the enhancement of Notch signaling by LFNG, whereas Gal inhibits the enhancement of DLL1-induced NOTCH signaling by MFNG Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
genes lfng and mfng, expression in wild-type CHO cells, in Lec1.3C, Lec2.6A, Lec8.3D, and Lec20.15C CHO glycosylation mutant lines, and in Lec8 and Lec20 CHO mutant lines Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information a DDD to DDA mutation inactivates MFNG GlcNAc transferase activity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
D-galactose Gal is required for the enhancement of Notch signaling by LFNG, whereas Gal inhibits the enhancement of DLL1-induced NOTCH signaling by MFNG Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus LFNG, Lunatic Fringe, and MFNG, Manic Fringe, transfer N-acetylglucosamine (GlcNAc) to O-fucose attached to EGF-like repeats of NOTCH receptors ?
-
?
[Notch]-fucose + UDP-alpha-D-N-acetylglucosamine Mus musculus
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[Notch]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
genes lfng and mfng
-

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information LFNG, Lunatic Fringe, and MFNG, Manic Fringe, transfer N-acetylglucosamine (GlcNAc) to O-fucose attached to EGF-like repeats of NOTCH receptors Mus musculus ?
-
?
[Notch]-fucose + UDP-alpha-D-N-acetylglucosamine
-
Mus musculus [Notch]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP
-
?

Synonyms

Synonyms Comment Organism
LFNG
-
Mus musculus
Lunatic Fringe
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Mus musculus
Manic fringe
-
Mus musculus
MFng
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Mus musculus

General Information

General Information Comment Organism
malfunction the NOTCH signaling effect through MFNG is abolished by a DDD to DDA mutation in MFNG that inactivates its GlcNAc transferase activity Mus musculus
physiological function LFNG and MFNG are both required for the optimal generation of MZB cells in spleen consistent with a synergistic action on Notch. Differential modification of EGF repeats on Notch by LFNG versus MFNG: MFNG promotes DLL1-induced NOTCH signaling better in the absence of Gal than in its presence, the effect is reversed in Lec8 cells corrected by expression of a UDP-Gal transporter cDNA. MFNG activity is required to enhance DLL1-induced Notch signaling in Lec8 cells. In co-culture Notch signaling assays, LFNG generally enhances DLL1-induced Notch signaling, but not in either Lec8 or Lec20 CHO mutants lacking Gal on O-fucose glycans. LFNG generally inhibits JAG1-induced Notch signaling, but in mutant CHO cells that do not add galactose to the GlcNAc transferred by Fringe, JAG1-induced Notch signaling is not inhibited by LFNG or MFNG, roles for Gal in LFNG and MFNG modulation of DLL1-induced Notch signaling in CHO cells, overview Mus musculus