Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-galactose | Gal is required for the enhancement of Notch signaling by LFNG, whereas Gal inhibits the enhancement of DLL1-induced NOTCH signaling by MFNG | Mus musculus |
Cloned (Comment) | Organism |
---|---|
genes lfng and mfng, expression in wild-type CHO cells, in Lec1.3C, Lec2.6A, Lec8.3D, and Lec20.15C CHO glycosylation mutant lines, and in Lec8 and Lec20 CHO mutant lines | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a DDD to DDA mutation inactivates MFNG GlcNAc transferase activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose | Gal is required for the enhancement of Notch signaling by LFNG, whereas Gal inhibits the enhancement of DLL1-induced NOTCH signaling by MFNG | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | LFNG, Lunatic Fringe, and MFNG, Manic Fringe, transfer N-acetylglucosamine (GlcNAc) to O-fucose attached to EGF-like repeats of NOTCH receptors | ? | - |
? | |
[Notch]-fucose + UDP-alpha-D-N-acetylglucosamine | Mus musculus | - |
[Notch]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
genes lfng and mfng | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | LFNG, Lunatic Fringe, and MFNG, Manic Fringe, transfer N-acetylglucosamine (GlcNAc) to O-fucose attached to EGF-like repeats of NOTCH receptors | Mus musculus | ? | - |
? | |
[Notch]-fucose + UDP-alpha-D-N-acetylglucosamine | - |
Mus musculus | [Notch]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LFNG | - |
Mus musculus |
Lunatic Fringe | - |
Mus musculus |
Manic fringe | - |
Mus musculus |
MFng | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | the NOTCH signaling effect through MFNG is abolished by a DDD to DDA mutation in MFNG that inactivates its GlcNAc transferase activity | Mus musculus |
physiological function | LFNG and MFNG are both required for the optimal generation of MZB cells in spleen consistent with a synergistic action on Notch. Differential modification of EGF repeats on Notch by LFNG versus MFNG: MFNG promotes DLL1-induced NOTCH signaling better in the absence of Gal than in its presence, the effect is reversed in Lec8 cells corrected by expression of a UDP-Gal transporter cDNA. MFNG activity is required to enhance DLL1-induced Notch signaling in Lec8 cells. In co-culture Notch signaling assays, LFNG generally enhances DLL1-induced Notch signaling, but not in either Lec8 or Lec20 CHO mutants lacking Gal on O-fucose glycans. LFNG generally inhibits JAG1-induced Notch signaling, but in mutant CHO cells that do not add galactose to the GlcNAc transferred by Fringe, JAG1-induced Notch signaling is not inhibited by LFNG or MFNG, roles for Gal in LFNG and MFNG modulation of DLL1-induced Notch signaling in CHO cells, overview | Mus musculus |