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Literature summary for 2.4.1.222 extracted from
- Eukaryotic expression, purification, crystallization and preliminary X-ray analysis of murine Manic Fringe (2006), Acta Crystallogr. Sect. F, 62, 774-777.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| catalytic domain of murine Manic Fringe is expressed in the baculovirus/insect-cell system as a secreted protein | Mus musculus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting-drop vapour diffusion method. Abolishing the glycosylation sites by site-directed mutagenesis was necessary in order to obtain orthorhombic crystals that diffracted to 1.8 A resolution. Crystals belonged to space group P2(1)2(1)2. The unit-cell parameters (a = 162.6, b = 41.4, c = 38.8 A) are consistent with the presence of one molecule in the asymmetric unit | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | O09008 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | presence of two N-linked glycans | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Manic fringe | - |
Mus musculus |
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