Literature summary for 2.4.1.220 extracted from

Hsu, T.M.; Welner, D.H.; Russ, Z.N.; Cervantes, B.; Prathuri, R.L.; Adams, P.D.; Dueber, J.E.
Employing a biochemical protecting group for a sustainable indigo dyeing strategy (2018), Nat. Chem. Biol., 14, 256-261 .

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Cloned(Commentary)

Cloned (Comment)	Organism
heterologous expression of PtUGT1 in Escherichia coli	Persicaria tinctoria

Crystallization (Commentary)

Crystallization (Comment)	Organism
the enzyme (PtUGT1) is cocrystallized with indoxyl sulfate in sitting drops consisting of 0.0002 ml protein solution and 0.0002 ml crystallization buffer. Crystal structure is solved at 2.14 A resolution with indoxyl sulfate, a glycosylation-incompetent indoxyl analog, in the active site	Persicaria tinctoria

Organism

Organism	UniProt	Comment	Textmining
Persicaria tinctoria	-	-	-
Persicaria tinctoria	A0A2R2JFJ4	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Persicaria tinctoria

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + indoxyl	-	Persicaria tinctoria	UDP + indican	-	?

Synonyms

Synonyms	Comment	Organism
PtUGT1	predominant isoform	Persicaria tinctoria
PtUGT2	differs from PtUGT1 by four amino acid residues V19M, G225A, E230Q, and A423D	Persicaria tinctoria
UDP-glucose:indoxyl glucosyltransferase	-	Persicaria tinctoria
UGIG	-	Persicaria tinctoria
UGT72B29	-	Persicaria tinctoria
UGT72B30	-	Persicaria tinctoria

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9.1	-	UDP-glucose	pH 7.8, 30°C	Persicaria tinctoria
12	-	UDP-glucose	pH 7.8, 30°C	Persicaria tinctoria

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Release 2023.1 (January 2023)