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Literature summary for 2.4.1.22 extracted from
- High-throughput mass-spectrometry monitoring for multisubstrate enzymes: determining the kinetic parameters and catalytic activities of glycosyltransferases (2005), CHEMBIOCHEM, 6, 346-357.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics for random and ordered bi bi mechanism, overview | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
recombinant enzyme, gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-alpha-D-galactose + D-glucose = UDP + lactose | random or ordered bi bi mechanism | Bos taurus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of a high-throughput mass spectrometry multisubstrate assay method | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-UDP-D-galactose + D-glucose | inversion of anomeric configuration to form beta-O-galactosides | Bos taurus | UDP + lactose | - |
? |  |
| alpha-UDP-D-galactose + N-acetyl-D-glucosamine | - |
Bos taurus | UDP + alpha-D-galactosyl(1-4)beta-N-acetyl-D-glucosamine | - |
? | |
| additional information | acceptor substrate specificity, overview | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, recombinant enzyme, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,4-galactosyltransferase | - |
Bos taurus |
| beta-GalT | - |
Bos taurus |
| GT | - |
Bos taurus |
| UGT72B1 | - |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bos taurus |
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Release 2023.1 (January 2023)
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