Application | Comment | Organism |
---|---|---|
synthesis | an artificial pathway is established in Escherichia coli for high-level production of arbutin from simple carbon sources in Escherichia coli for high-level production of arbutin from simple carbon sources. Introduction of the genes for 4-hydroxybenzoate 1-hydroxylase from Candida parapsilosis CBS604 and hydroquinone glucosyltransferase from Rauvolfia serpentina into Escherichia coli leads to the production of 54.71 mg/l of arbutin from glucose. Further redirection of carbon flux into arbutin biosynthesis pathway by enhancing shikimate pathway genes enables production of 3.29 g/l arbutin. Final optimization of glucose concentration added in the culture medium is able to further improve the titer of arbutin to 4.19 g/l in shake flasks experiments | Rauvolfia serpentina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
hydroquinone | pH 7.5, 30°C | Rauvolfia serpentina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | Q9AR73 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + hydroquinone | - |
Rauvolfia serpentina | UDP + hydroquinone-O-beta-D-glucopyranoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arbutin synthase | - |
Rauvolfia serpentina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00038 | - |
hydroquinone | pH 7.5, 30°C | Rauvolfia serpentina |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
hydroquinone | pH 7.5, 30°C | Rauvolfia serpentina |