Cloned (Comment) | Organism |
---|---|
phylogenetic analysis | Thermus thermophilus |
phylogenetic analysis | Rhodothermus marinus |
phylogenetic analysis | Pyrococcus horikoshii |
phylogenetic analysis | Rubrobacter xylanophilus |
phylogenetic analysis | Palaeococcus ferrophilus |
Crystallization (Comment) | Organism |
---|---|
enzyme in apo-form and in complex with GMP and GDP-mannose | Rubrobacter xylanophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
3-phospho-D-glycerate | pH 7.0, 80-90°C | Thermus thermophilus | |
0.14 | - |
3-phospho-D-glycerate | pH 6.4-7.4, 90°C | Pyrococcus horikoshii | |
0.17 | - |
GDP-mannose | pH 6.4-7.4, 90°C | Pyrococcus horikoshii | |
0.26 | - |
GDP-mannose | pH 7.0-8.0, 70-75°C | Rubrobacter xylanophilus | |
0.33 | - |
GDP-mannose | pH 7.0, 80-90°C | Thermus thermophilus | |
0.34 | - |
3-phospho-D-glycerate | pH 7.0-8.0, 70-75°C | Rubrobacter xylanophilus | |
0.5 | - |
GDP-mannose | pH 7.5, 80°C | Rhodothermus marinus | |
0.63 | - |
3-phospho-D-glycerate | pH 7.5, 80°C | Rhodothermus marinus | |
1.21 | - |
GDP-glucose | pH 7.0-8.0, 70-75°C | Rubrobacter xylanophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binding structure overview | Thermus thermophilus | |
Mg2+ | binding structure overview | Rhodothermus marinus | |
Mg2+ | binding structure overview | Pyrococcus horikoshii | |
Mg2+ | binding structure overview | Rubrobacter xylanophilus | |
Mg2+ | binding structure overview | Palaeococcus ferrophilus | |
Mn2+ | binding structure overview | Thermus thermophilus | |
Mn2+ | binding structure overview | Rhodothermus marinus | |
Mn2+ | binding structure overview | Pyrococcus horikoshii | |
Mn2+ | binding structure overview | Rubrobacter xylanophilus | |
Mn2+ | binding structure overview | Palaeococcus ferrophilus | |
Zn2+ | binding structure overview | Thermus thermophilus | |
Zn2+ | binding structure overview | Rhodothermus marinus | |
Zn2+ | binding structure overview | Pyrococcus horikoshii | |
Zn2+ | binding structure overview | Rubrobacter xylanophilus | |
Zn2+ | binding structure overview | Palaeococcus ferrophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-glucose + 3-phospho-D-glycerate | Rubrobacter xylanophilus | - |
GDP + 2-(alpha-D-glucosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | Thermus thermophilus | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | Rhodothermus marinus | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | Pyrococcus horikoshii | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | Rubrobacter xylanophilus | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | Palaeococcus ferrophilus | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | Pyrococcus horikoshii OT-3 | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
additional information | Rhodothermus marinus | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate | ? | - |
? | |
additional information | Pyrococcus horikoshii | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate | ? | - |
? | |
additional information | Palaeococcus ferrophilus | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate | ? | - |
? | |
additional information | Thermus thermophilus | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate. Substrate binding structure, overview | ? | - |
? | |
additional information | Rubrobacter xylanophilus | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate. The enzyme from Rubrobacter xylanophilus is promiscuous and produces the phosphorylated form of glucosylglycerate (GPG) from GDP-glucose plus 3-D-phosphoglycerate with high efficiency. In spite of the less favorable parameters for the synthesis of mannosylglycerate, this is the only free glyceryl glycoside found in Rubrobacter xylanophilus cells | ? | - |
? | |
additional information | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate. Substrate binding structure, overview | ? | - |
? | |
additional information | Pyrococcus horikoshii OT-3 | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Palaeococcus ferrophilus | - |
- |
- |
Pyrococcus horikoshii | - |
- |
- |
Pyrococcus horikoshii OT-3 | - |
- |
- |
Rhodothermus marinus | - |
- |
- |
Rubrobacter xylanophilus | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | front-face SNi-like reaction mechanism, detailed overview. The binding of GDP-mannose:Mn2+ to the enzyme from Rhodothermus marinus induces significant conformational changes in the flexible loop. In particular, Tyr220 plays a pivotal role both in D-glycerate binding and in catalysis: it is reoriented towards the pocket interior and either interacts with the a phosphate of GDP-mannose | Rhodothermus marinus | |
GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | front-face SNi-like reaction mechanism, overview | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-glucose + 3-phospho-D-glycerate | - |
Rubrobacter xylanophilus | GDP + 2-(alpha-D-glucosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | - |
Thermus thermophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | - |
Rhodothermus marinus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | - |
Pyrococcus horikoshii | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | - |
Rubrobacter xylanophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | - |
Palaeococcus ferrophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | - |
Pyrococcus horikoshii OT-3 | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
additional information | substrate binding structure, overview | Pyrococcus horikoshii | ? | - |
? | |
additional information | substrate binding structure, overview | Rubrobacter xylanophilus | ? | - |
? | |
additional information | substrate binding structure, overview | Palaeococcus ferrophilus | ? | - |
? | |
additional information | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate | Rhodothermus marinus | ? | - |
? | |
additional information | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate | Pyrococcus horikoshii | ? | - |
? | |
additional information | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate | Palaeococcus ferrophilus | ? | - |
? | |
additional information | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate. Substrate binding structure, overview | Thermus thermophilus | ? | - |
? | |
additional information | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate. The enzyme from Rubrobacter xylanophilus is promiscuous and produces the phosphorylated form of glucosylglycerate (GPG) from GDP-glucose plus 3-D-phosphoglycerate with high efficiency. In spite of the less favorable parameters for the synthesis of mannosylglycerate, this is the only free glyceryl glycoside found in Rubrobacter xylanophilus cells | Rubrobacter xylanophilus | ? | - |
? | |
additional information | besides its physiological substrate D-glycerate, RmaMGS is also able to use D-lactate and glycolate as sugar acceptors, thus displaying some acceptor plasticity. Substrate binding structure, overview | Rhodothermus marinus | ? | - |
? | |
additional information | the enzyme is highly specific for 3-phospho-D-glycerate | Thermus thermophilus | ? | - |
? | |
additional information | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate. Substrate binding structure, overview | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? | |
additional information | the enzyme is highly specific for 3-phospho-D-glycerate | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? | |
additional information | substrate binding structure, overview | Pyrococcus horikoshii OT-3 | ? | - |
? | |
additional information | all MPGSs have high affinity for GDP-mannose and 3-D-phosphoglycerate | Pyrococcus horikoshii OT-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | monomer structure from crystal structure modeling, overview | Thermus thermophilus |
More | monomer structure from crystal structure modeling, overview | Rhodothermus marinus |
More | monomer structure from crystal structure modeling, overview | Pyrococcus horikoshii |
More | monomer structure from crystal structure modeling, overview | Rubrobacter xylanophilus |
More | monomer structure from crystal structure modeling, overview | Palaeococcus ferrophilus |
Synonyms | Comment | Organism |
---|---|---|
MPGS | - |
Thermus thermophilus |
MPGS | - |
Rhodothermus marinus |
MPGS | - |
Pyrococcus horikoshii |
MPGS | - |
Palaeococcus ferrophilus |
MPGS/GPGS | - |
Rubrobacter xylanophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 75 | - |
Rubrobacter xylanophilus |
80 | 90 | - |
Thermus thermophilus |
80 | 90 | - |
Rhodothermus marinus |
90 | - |
- |
Palaeococcus ferrophilus |
90 | 100 | - |
Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life is 23-28 min dependent on the substrate | Rubrobacter xylanophilus |
80 | - |
half-life is 40 min | Rhodothermus marinus |
80 | - |
half-life is 189 min | Thermus thermophilus |
83 | - |
half-life is 18 min | Palaeococcus ferrophilus |
90 | - |
half-life is 22 min | Thermus thermophilus |
98 | - |
half-life is 16 min | Pyrococcus horikoshii |
100 | - |
half-life is 10 min | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.4 | - |
Pyrococcus horikoshii |
7 | 8 | - |
Rubrobacter xylanophilus |
7 | - |
- |
Thermus thermophilus |
7 | - |
- |
Palaeococcus ferrophilus |
7.5 | - |
- |
Rhodothermus marinus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is included in the glycosyltransferase family GT55 | Thermus thermophilus |
evolution | the enzyme is included in the glycosyltransferase family GT55 | Rhodothermus marinus |
evolution | the enzyme is included in the glycosyltransferase family GT55 | Pyrococcus horikoshii |
evolution | the enzyme is included in the glycosyltransferase family GT55 | Palaeococcus ferrophilus |
evolution | the promiscuous MPGS/GPGS from Rubrobacter xylanophilus (RxyMPGS) is included within the retaining GT81 family, members of the GT55 and GT81 families preserved a common structural core, defined by the alpha/beta/alpha region containing 7 beta-strands in the order 3-2-1-4-6-5-7, with beta6 antiparallel to the rest, that could be included into the MGS-like family | Rubrobacter xylanophilus |
metabolism | mannosylglycerate metabolism overview: pathways for the synthesis and hydrolysis of mannosylglycerate (MG). In the single-step pathway mannosylglycerate synthase (MGS) catalyzes the direct condensation of GDP-mannose with D-glycerate to produce MG. In the two-step pathway, mannosyl-3-phosphoglycerate synthase (MPGS) catalyzes the conversion of GDP-mannose and D-3-phosphoglycerate into mannosyl-3-phosphoglycerate (MPG) which is dephosphorylated into MG by mannosyl-3-phosphoglycerate phosphatase (MPGP) | Thermus thermophilus |
metabolism | mannosylglycerate metabolism overview: pathways for the synthesis and hydrolysis of mannosylglycerate (MG). In the single-step pathway mannosylglycerate synthase (MGS) catalyzes the direct condensation of GDP-mannose with D-glycerate to produce MG. In the two-step pathway, mannosyl-3-phosphoglycerate synthase (MPGS) catalyzes the conversion of GDP-mannose and D-3-phosphoglycerate into mannosyl-3-phosphoglycerate (MPG) which is dephosphorylated into MG by mannosyl-3-phosphoglycerate phosphatase (MPGP) | Rhodothermus marinus |
metabolism | mannosylglycerate metabolism overview: pathways for the synthesis and hydrolysis of mannosylglycerate (MG). In the single-step pathway mannosylglycerate synthase (MGS) catalyzes the direct condensation of GDP-mannose with D-glycerate to produce MG. In the two-step pathway, mannosyl-3-phosphoglycerate synthase (MPGS) catalyzes the conversion of GDP-mannose and D-3-phosphoglycerate into mannosyl-3-phosphoglycerate (MPG) which is dephosphorylated into MG by mannosyl-3-phosphoglycerate phosphatase (MPGP) | Pyrococcus horikoshii |
metabolism | mannosylglycerate metabolism overview: pathways for the synthesis and hydrolysis of mannosylglycerate (MG). In the single-step pathway mannosylglycerate synthase (MGS) catalyzes the direct condensation of GDP-mannose with D-glycerate to produce MG. In the two-step pathway, mannosyl-3-phosphoglycerate synthase (MPGS) catalyzes the conversion of GDP-mannose and D-3-phosphoglycerate into mannosyl-3-phosphoglycerate (MPG) which is dephosphorylated into MG by mannosyl-3-phosphoglycerate phosphatase (MPGP) | Palaeococcus ferrophilus |
metabolism | mannosylglycerate metabolism overview: pathways for the synthesis and hydrolysis of mannosylglycerate (MG). In the single-step pathway mannosylglycerate synthase (MGS)catalyzes the direct condensation of GDP-mannose with D-glycerate to produce MG. In the two-step pathway, mannosyl-3-phosphoglycerate synthase (MPGS) catalyzes the conversion of GDP-mannose and D-3-phosphoglycerate into mannosyl-3-phosphoglycerate (MPG) which is dephosphorylated into MG by mannosyl-3-phosphoglycerate phosphatase (MPGP) | Rubrobacter xylanophilus |
additional information | the catalytic pocket of MPGS is solvent-exposed at the NDP-sugar binding region, allowing ready access of the substrate | Thermus thermophilus |
additional information | the catalytic pocket of MPGS is solvent-exposed at the NDP-sugar binding region, allowing ready access of the substrate | Rhodothermus marinus |
additional information | the catalytic pocket of MPGS is solvent-exposed at the NDP-sugar binding region, allowing ready access of the substrate | Pyrococcus horikoshii |
additional information | the catalytic pocket of MPGS is solvent-exposed at the NDP-sugar binding region, allowing ready access of the substrate | Rubrobacter xylanophilus |
additional information | the catalytic pocket of MPGS is solvent-exposed at the NDP-sugar binding region, allowing ready access of the substrate | Palaeococcus ferrophilus |