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Literature summary for 2.4.1.217 extracted from
- Functional and structural characterization of a novel mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus reveals its dual substrate specificity (2011), Mol. Microbiol., 79, 76-93.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| corresponding gene is expressed in Escherichia coli | Rubrobacter xylanophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure of the unliganded MpgS and of its complexes with the nucleotide and sugar donors, at 2.2, 2.8 and 2.5 A resolution respectively | Rubrobacter xylanophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A164S/A208S/F211Y | MpgS triple mutant engineered to reinforce the hydrogen-bonding network | Rubrobacter xylanophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
GDP-mannose | wild-type | Rubrobacter xylanophilus |  |
| 0.34 | - |
3-phospho-D-glycerate | wild-type, with GDP-mannose | Rubrobacter xylanophilus | |
| 0.49 | - |
GDP-mannose | A164S/A208S/F211Y mutant | Rubrobacter xylanophilus | |
| 0.81 | - |
3-phospho-D-glycerate | A164S/A208S/F211Y mutant, with GDP-mannose | Rubrobacter xylanophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ | Rubrobacter xylanophilus | |
| Mg2+ | strictly dependent on, effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ | Rubrobacter xylanophilus | |
| Mn2+ | effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ | Rubrobacter xylanophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rubrobacter xylanophilus | B7SY86 | DSM 9941 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified from cell extracts | Rubrobacter xylanophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0232 | - |
with GDP-mannose as a substrate | Rubrobacter xylanophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose + 3-phospho-D-glycerate | - |
Rubrobacter xylanophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mannosyl-3-phosphoglycerate synthase | - |
Rubrobacter xylanophilus |
| mannosyl-3-phosphoglycerate synthase/phosphatase | - |
Rubrobacter xylanophilus |
| MPGS | GT81 family | Rubrobacter xylanophilus |
| MpgS/P | - |
Rubrobacter xylanophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 75 | - |
Rubrobacter xylanophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme is thermophilic | Rubrobacter xylanophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Rubrobacter xylanophilus |
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