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Literature summary for 2.4.1.217 extracted from

  • Sa-Moura, B.; Albuquerque, L.; Empadinhas, N.; da Costa, M.S.; Pereira, P.J.; Macedo-Ribeiro, S.
    Crystallization and preliminary crystallographic analysis of mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus (2008), Acta Crystallogr. Sect. F, 64, 760-763.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.217

Cloned(Commentary)

Cloned (Comment) Organism
into the pGEMT-Easy vector for sequencing and subcloned into pET30a for expression in Escherichia coli BL21DE3 cells Rubrobacter xylanophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
for the native protein a data set extending to 2.20 A resolution is collected, for the mercury derivative a data set extending to 3.05 A, and for the ternary complex with GDP and mannose a data set extending to 2.80 A Rubrobacter xylanophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 recombinant fusion protein, determined by SDS-PAGE Rubrobacter xylanophilus
95000
-
 determined by gel filtration, suggesting that it is dimeric in solution Rubrobacter xylanophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-mannose + 3-phospho-D-glycerate Rubrobacter xylanophilus
-
 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Rubrobacter xylanophilus B7SY86
-
-

Purification (Commentary)

Purification (Comment) Organism
native mannosyl-3-phosphoglycerate synthase is purified from Rubrobacter xylanophilus cells using a Q-Sepharose FF and a Mono-Q column, recombinant MpgS is purified by immobilized metal-affinity chromatography on a Ni-Sepharose column Rubrobacter xylanophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + 3-phospho-D-glycerate
-
 Rubrobacter xylanophilus GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?

Synonyms

Synonyms Comment Organism
mannosyl-3-phosphoglycerate synthase
-
 Rubrobacter xylanophilus
MPGS
-
 Rubrobacter xylanophilus