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Literature summary for 2.4.1.217 extracted from
- Compatible solutes of the hyperthermophile Palaeococcus ferrophilus: osmoadaptation and thermoadaptation in the order Thermococcales (2005), Appl. Environ. Microbiol., 71, 8091-8098.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Palaeococcus ferrophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strictly dependent on Mg2+ | Palaeococcus ferrophilus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44264 | - |
x * 44264, calculated from sequence | Palaeococcus ferrophilus |
| 44300 | - |
x * 44300, SDS-PAGE | Palaeococcus ferrophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose + 3-phospho-D-glycerate | Palaeococcus ferrophilus | synthesis of mannosylglycerate proceeds via the two-step pathway comprising mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70) | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | Thermococcus litoralis | synthesis of mannosylglycerate proceeds via the two-step pathway comprising mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70) | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | Palaeococcus ferrophilus | the enzyme is involved in synthesis of mannosylglycerate | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | Palaeococcus ferrophilus DSM 13482T | synthesis of mannosylglycerate proceeds via the two-step pathway comprising mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70) | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | Palaeococcus ferrophilus DSM 13482T | the enzyme is involved in synthesis of mannosylglycerate | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Palaeococcus ferrophilus | Q2VPS1 | - |
- |
| Palaeococcus ferrophilus DSM 13482T | Q2VPS1 | - |
- |
| Thermococcus litoralis | Q2VPR9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Palaeococcus ferrophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-mannose + 3-phospho-D-glycerate | - |
Palaeococcus ferrophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | - |
Thermococcus litoralis | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | synthesis of mannosylglycerate proceeds via the two-step pathway comprising mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70) | Palaeococcus ferrophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | synthesis of mannosylglycerate proceeds via the two-step pathway comprising mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70) | Thermococcus litoralis | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | the enzyme is involved in synthesis of mannosylglycerate | Palaeococcus ferrophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | the enzyme is highly specific for the substrates GDP-mannose and 3-phospho-D-glycerate | Palaeococcus ferrophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | - |
Palaeococcus ferrophilus DSM 13482T | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | synthesis of mannosylglycerate proceeds via the two-step pathway comprising mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70) | Palaeococcus ferrophilus DSM 13482T | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | the enzyme is involved in synthesis of mannosylglycerate | Palaeococcus ferrophilus DSM 13482T | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
| GDP-mannose + 3-phospho-D-glycerate | the enzyme is highly specific for the substrates GDP-mannose and 3-phospho-D-glycerate | Palaeococcus ferrophilus DSM 13482T | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44264, calculated from sequence | Palaeococcus ferrophilus |
| ? | x * 44300, SDS-PAGE | Palaeococcus ferrophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MPGS | - |
Palaeococcus ferrophilus |
| MPGS | - |
Thermococcus litoralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Palaeococcus ferrophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | 80°C: about 60% of maximal activity, 100°C: about 60% of maximal activity | Palaeococcus ferrophilus |
| 80 | 100 | 80°C: about 55% of maximal activity, 100°C: about 60% of maximal activity | Palaeococcus ferrophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 83 | - |
half-life: 18 min | Palaeococcus ferrophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Palaeococcus ferrophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | pH 6.0: about 40% of maximal activity, pH 8.0: about 40% of maximal activity | Palaeococcus ferrophilus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in synthesis of mannosylglycerate | Palaeococcus ferrophilus |
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