Cloned (Comment) | Organism |
---|---|
gene mgsD, DNA and amino acid sequence determination and analysis, genetic organization, overexpression of the holoenzyme and of the isolated mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase domains of the bifunctional enzyme in Escherichia coli, the recombinant Escherichia coli strain does not accumulate mannosylglycerate, expression of active bifunctional enzyme in Saccharomyces cerevisiae | Dehalococcoides mccartyi |
General Stability | Organism |
---|---|
recombinant monofunctional mannosyl-3-phosphoglycerate synthase domain is unstable | Dehalococcoides mccartyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
GDP-mannose | pH 7.5, 30°C, recombinant holoenzyme | Dehalococcoides mccartyi | |
2.18 | - |
3-phospho-D-glycerate | pH 7.5, 30°C, recombinant holoenzyme | Dehalococcoides mccartyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | most effective in enzyme activation | Dehalococcoides mccartyi | |
Mg2+ | activates | Dehalococcoides mccartyi | |
Mn2+ | activates | Dehalococcoides mccartyi | |
additional information | enzyme requires divalent cations, Co2+ is preferred and can partially be substituted by Mg2+ or Mn2+, no activation by other cations | Dehalococcoides mccartyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 78000, recombinant bifunctional holoenzyme, SDS-PAGE, x * 50000, about, recombinant mannosyl-3-phosphoglycerate synthase domain, SDS-PAGE | Dehalococcoides mccartyi |
78000 | - |
x * 78000, recombinant bifunctional holoenzyme, SDS-PAGE, x * 50000, about, recombinant mannosyl-3-phosphoglycerate synthase domain, SDS-PAGE | Dehalococcoides mccartyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + 3-phospho-D-glycerate | Dehalococcoides mccartyi | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
additional information | Dehalococcoides mccartyi | the bifunctional enzyme catalyzes the consecutive synthesis and dephosphorylation of mannosyl-3-phosphoglycerate in mannosylglycerate biosynthesis, enzyme evolution | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dehalococcoides mccartyi | - |
gene mgsD, bifunctional enzyme | - |
no activity in Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme and enzyme domains from Escherichia coli | Dehalococcoides mccartyi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | the enzyme is bifunctional exhibiting both mannosyl-3-phosphoglycerate synthase, EC 2.4.1.217, and mannosyl-3-phosphoglycerate phosphatase, EC 3.1.3.70, activities, catalyzing the consecutive synthesis and dephosphorylation of mannosyl-3-phosphoglycerate in mannosylglycerate biosynthesis | Dehalococcoides mccartyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + 3-phospho-D-glycerate | - |
Dehalococcoides mccartyi | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
GDP-mannose + 3-phospho-D-glycerate | the enzyme is absolute specific for GDP-mannose and 3-phospho-D-glycerate as substrates | Dehalococcoides mccartyi | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? | |
additional information | the bifunctional enzyme catalyzes the consecutive synthesis and dephosphorylation of mannosyl-3-phosphoglycerate in mannosylglycerate biosynthesis, enzyme evolution | Dehalococcoides mccartyi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, recombinant bifunctional holoenzyme, SDS-PAGE, x * 50000, about, recombinant mannosyl-3-phosphoglycerate synthase domain, SDS-PAGE | Dehalococcoides mccartyi |
Synonyms | Comment | Organism |
---|---|---|
mannosylglycerate synthase | - |
Dehalococcoides mccartyi |
MGSD | - |
Dehalococcoides mccartyi |
MPGS | mannosyl-3-phosphoglycerate synthase domain of the bifunctional enzyme | Dehalococcoides mccartyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Dehalococcoides mccartyi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | temperature-profile, recombinant enzyme | Dehalococcoides mccartyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
3-phospho-D-glycerate | pH 7.5, 30°C, recombinant holoenzyme | Dehalococcoides mccartyi | |
3.9 | - |
GDP-mannose | pH 7.5, 30°C, recombinant holoenzyme | Dehalococcoides mccartyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Dehalococcoides mccartyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | pH-profile | Dehalococcoides mccartyi |