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Literature summary for 2.4.1.217 extracted from

  • Empadinhas, N.; Albuquerque, L.; Henne, A.; Santos, H.; da Costa, M.S.
    The bacterium Thermus thermophilus, like hyperthermophilic archaea, uses a two-step pathway for the synthesis of mannosylglycerate (2003), Appl. Environ. Microbiol., 69, 3272-3279.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.217

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, genetic organization of genes involved in mannosylglycerate biosynthesis, functional overexpression in Escherichia coli strain BL21 Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
KCl inhibitory at 50-300 mM Thermus thermophilus
NaCl inhibitory at 50-300 mM Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 3-phospho-D-glycerate pH 8.0, 80°C, recombinant enzyme Thermus thermophilus
0.33
-
 GDP-mannose pH 8.0, 80°C, recombinant enzyme Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, best at 2 mM Thermus thermophilus
Mg2+ activates, best at 20 mM Thermus thermophilus
Mn2+ Mn2+ is most effective in enzyme activation, best at 1 mM Thermus thermophilus
additional information strictly dependent on divalent cations, no activation by Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+ Thermus thermophilus
Ni2+ activates, best at 1.5 mM Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, recombinant enzyme, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-mannose + 3-phospho-D-glycerate Thermus thermophilus
-
 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?
GDP-mannose + 3-phospho-D-glycerate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q84B24 AY193871, GenBank sequence encoding mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q84B24 AY193871, GenBank sequence encoding mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + 3-phospho-D-glycerate
-
 Thermus thermophilus GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?
GDP-mannose + 3-phospho-D-glycerate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, recombinant enzyme, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
MPGS
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 90 recombinant enzyme Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 100 temperature-profile, 10% of maximal activity at 25°C, 55% of maximal activity at 103°C Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life 189 min Thermus thermophilus
90
-
 half-life 22 min Thermus thermophilus
100
-
 half-life 10 min Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 about, recombinant enzyme Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH-profile, 30-40% of maximal activity at pH 5.0 and pH 9.0 Thermus thermophilus