BRENDA - Enzyme Database
show all sequences of 2.4.1.216

Efficient one-pot enzymatic synthesis of trehalose 6-phosphate using GH65 alpha-glucoside phosphorylases

Taguchi, Y.; Saburi, W.; Imai, R.; Mori, H.; Carbohydr. Res. 488, 107902 (2020) View publication on PubMed

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
efficient one-pot enzymatic synthesis of trehalose 6-phosphate. The one-pot reaction of trehalose 6-phosphate phosphorylase and maltose phosphorylase enables production of 65 mM trehalose 6-phosphate from 100 mM maltose, 100 mM Glc6P, and 20 mM inorganic phosphate. Addition of beta-phosphoglucomutase to this reaction produces glucose 6-phosphate from beta-glucose 1-phosphate and thus reduces requirement of glucose 6-phosphate as a starting material. Within the range of 20-469 mM inorganic phosphate tested, the 54 mM concentration yielded the highest amount of trehalose 6-phosphate (33 mM). Addition of yeast increases the yield because of its glucose consumption. From 100 mmol maltose and 60 mmol inorganic phosphate, the production of 37.5 mmol trehalose 6-phosphate in a one-pot reaction (100 ml) is achieved, and 9.4 g trehalose 6-phosphate dipotassium salt is obtained
Lactococcus lactis subsp. lactis
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Lactococcus lactis subsp. lactis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glucose 6-phosphate + beta-D-glucose 1-phosphate
Lactococcus lactis subsp. lactis
trehalose 6-phosphate is an important intermediate for trehalose biosynthesis. Trehalose 6-phosphate is an endogenous signaling molecule that regulates plant development and stress responses
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Lactococcus lactis subsp. lactis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
759004
Lactococcus lactis subsp. lactis
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
-
r
glucose 6-phosphate + beta-D-glucose 1-phosphate
trehalose 6-phosphate is an important intermediate for trehalose biosynthesis. Trehalose 6-phosphate is an endogenous signaling molecule that regulates plant development and stress responses
759004
Lactococcus lactis subsp. lactis
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
TrePP
-
Lactococcus lactis subsp. lactis
Application (protein specific)
Application
Commentary
Organism
synthesis
efficient one-pot enzymatic synthesis of trehalose 6-phosphate. The one-pot reaction of trehalose 6-phosphate phosphorylase and maltose phosphorylase enables production of 65 mM trehalose 6-phosphate from 100 mM maltose, 100 mM Glc6P, and 20 mM inorganic phosphate. Addition of beta-phosphoglucomutase to this reaction produces glucose 6-phosphate from beta-glucose 1-phosphate and thus reduces requirement of glucose 6-phosphate as a starting material. Within the range of 20-469 mM inorganic phosphate tested, the 54 mM concentration yielded the highest amount of trehalose 6-phosphate (33 mM). Addition of yeast increases the yield because of its glucose consumption. From 100 mmol maltose and 60 mmol inorganic phosphate, the production of 37.5 mmol trehalose 6-phosphate in a one-pot reaction (100 ml) is achieved, and 9.4 g trehalose 6-phosphate dipotassium salt is obtained
Lactococcus lactis subsp. lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Lactococcus lactis subsp. lactis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glucose 6-phosphate + beta-D-glucose 1-phosphate
Lactococcus lactis subsp. lactis
trehalose 6-phosphate is an important intermediate for trehalose biosynthesis. Trehalose 6-phosphate is an endogenous signaling molecule that regulates plant development and stress responses
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
759004
Lactococcus lactis subsp. lactis
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
-
r
glucose 6-phosphate + beta-D-glucose 1-phosphate
trehalose 6-phosphate is an important intermediate for trehalose biosynthesis. Trehalose 6-phosphate is an endogenous signaling molecule that regulates plant development and stress responses
759004
Lactococcus lactis subsp. lactis
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
-
r
Other publictions for EC 2.4.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
759004
Taguchi
Efficient one-pot enzymatic s ...
Lactococcus lactis subsp. lactis
Carbohydr. Res.
488
107902
2020
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758932
Taguchi
Evaluation of acceptor select ...
Lactococcus lactis ssp. lactis, Lactococcus lactis ssp. lactis JCM 5805
Biosci. Biotechnol. Biochem.
81
1512-1519
2017
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1
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5
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1
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6
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6
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1
1
-
2
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736176
Shi
Trehalose metabolism in the bl ...
Callinectes sapidus
Gene
536
105-113
2014
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1
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721344
Carvalho
Engineering trehalose synthesi ...
Lactococcus lactis, Lactococcus lactis MG1363
Appl. Environ. Microbiol.
77
4189-4199
2011
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-
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1
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8
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395984
Andersson U.; Radström
beta-Glucose 1-phosphate-inter ...
Carnobacterium divergens, Carnobacterium maltaromaticum, Carnobacterium sp., Enterococcus faecalis, Enterococcus faecium, Lactiplantibacillus plantarum, Lactobacillus casei ssp. casei, Lactobacillus delbrueckii ssp. delbrueckii, Lactiplantibacillus pentosus, Limosilactobacillus reuteri, Weissella viridescens, Lactococcus lactis, Lactococcus lactis ssp. cremoris, Lactococcus lactis ssp. lactis, Lactococcus plantarum, Lactococcus raffinolactis, Leuconostoc mesenteroides ssp. dextranicum, Oenococcus oeni, Leuconostoc pseudomesenteroides, Leuconostoc weissella, Pediococcus acidilactici, Pediococcus damnosus, Tetragenococcus halophilus, Pediococcus pentosaceus, Vagococcus salmoninarum, Carnobacterium sp. 496
Environ. Microbiol.
4
81-88
2002
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26
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30
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25
2
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26
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26
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25
2
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26
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395985
Andersson
Trehalose-6-phosphate phosphor ...
Lactococcus lactis, Lactococcus lactis IL1403
J. Biol. Chem.
276
42707-42713
2001
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4
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1
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10
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1
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2
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2
1
1
1
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1
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1
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4
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1
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1
1
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1
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