BRENDA - Enzyme Database
show all sequences of 2.4.1.216

Evaluation of acceptor selectivity of Lactococcus lactis ssp. lactis trehalose 6-phosphate phosphorylase in the reverse phosphorolysis and synthesis of a new sugar phosphate

Taguchi, Y.; Saburi, W.; Imai, R.; Mori, H.; Biosci. Biotechnol. Biochem. 81, 1512-1519 (2017) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Lactococcus lactis ssp. lactis
Inhibitors
Inhibitors
Commentary
Organism
Structure
beta-D-glucose 1-phosphate
-
Lactococcus lactis ssp. lactis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.413
-
beta-D-glucose 1-phosphate
pH 5.5, 30°C
Lactococcus lactis ssp. lactis
1.85
-
glucose 6-phosphate
pH 5.5, 30°C
Lactococcus lactis ssp. lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
88387
-
calculated from sequence
Lactococcus lactis ssp. lactis
90000
-
SDS-PAGE
Lactococcus lactis ssp. lactis
184000
-
blue native PAGE
Lactococcus lactis ssp. lactis
Organism
Organism
UniProt
Commentary
Textmining
Lactococcus lactis ssp. lactis
-
-
-
Lactococcus lactis ssp. lactis JCM 5805
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Lactococcus lactis ssp. lactis
Storage Stability
Storage Stability
Organism
4°C, activity of is retained after incubation at pH 4.5-9.0 for 24 h
Lactococcus lactis ssp. lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
alpha,alpha-trehalose 6-phosphate + phosphate
-
758932
Lactococcus lactis ssp. lactis
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose 6-phosphate + phosphate
-
758932
Lactococcus lactis ssp. lactis JCM 5805
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
-
-
r
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
758932
Lactococcus lactis ssp. lactis
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
-
r
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
758932
Lactococcus lactis ssp. lactis JCM 5805
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
-
r
mannose 6-phosphate + beta-D-glucose 1-phosphate
mannose 6-phosphate is a poor substrate. Relative activity compared to glucose 6-phosphate is 0.05%
758932
Lactococcus lactis ssp. lactis
alpha-D-Glc-(1->1)-alpha-D-Man6P + phosphate
-
-
-
?
mannose 6-phosphate + beta-D-glucose 1-phosphate
mannose 6-phosphate is a poor substrate. Relative activity compared to glucose 6-phosphate is 0.05%
758932
Lactococcus lactis ssp. lactis JCM 5805
alpha-D-Glc-(1->1)-alpha-D-Man6P + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 90000, SDS-PAGE
Lactococcus lactis ssp. lactis
Synonyms
Synonyms
Commentary
Organism
TrePP
-
Lactococcus lactis ssp. lactis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Lactococcus lactis ssp. lactis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
50
25°C: about 45% of maximal activity, 50°C: about 80% of maximal activity
Lactococcus lactis ssp. lactis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
activity is retained after incubation at pH 5.0-8.0 at 30°C for 15 min
Lactococcus lactis ssp. lactis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
759
-
glucose 6-phosphate
pH 5.5, 30°C
Lactococcus lactis ssp. lactis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.8
-
synthesis of alpha,alpha-trehalose 6-phosphate
Lactococcus lactis ssp. lactis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4.5
9
activity is retained after incubation at pH 4.5-9.0 at 4°C for 24 h
Lactococcus lactis ssp. lactis
5
8
activity is retained after incubation at pH 5.0-8.0 at 30°C for 15 min
Lactococcus lactis ssp. lactis
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.519
-
beta-D-glucose 1-phosphate
pH 5.5, 30°C
Lactococcus lactis ssp. lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Lactococcus lactis ssp. lactis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
beta-D-glucose 1-phosphate
-
Lactococcus lactis ssp. lactis
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.519
-
beta-D-glucose 1-phosphate
pH 5.5, 30°C
Lactococcus lactis ssp. lactis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.413
-
beta-D-glucose 1-phosphate
pH 5.5, 30°C
Lactococcus lactis ssp. lactis
1.85
-
glucose 6-phosphate
pH 5.5, 30°C
Lactococcus lactis ssp. lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
88387
-
calculated from sequence
Lactococcus lactis ssp. lactis
90000
-
SDS-PAGE
Lactococcus lactis ssp. lactis
184000
-
blue native PAGE
Lactococcus lactis ssp. lactis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lactococcus lactis ssp. lactis
Storage Stability (protein specific)
Storage Stability
Organism
4°C, activity of is retained after incubation at pH 4.5-9.0 for 24 h
Lactococcus lactis ssp. lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
alpha,alpha-trehalose 6-phosphate + phosphate
-
758932
Lactococcus lactis ssp. lactis
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
-
-
r
alpha,alpha-trehalose 6-phosphate + phosphate
-
758932
Lactococcus lactis ssp. lactis JCM 5805
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
-
-
r
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
758932
Lactococcus lactis ssp. lactis
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
-
r
glucose 6-phosphate + beta-D-glucose 1-phosphate
-
758932
Lactococcus lactis ssp. lactis JCM 5805
alpha,alpha-trehalose 6-phosphate + phosphate
-
-
-
r
mannose 6-phosphate + beta-D-glucose 1-phosphate
mannose 6-phosphate is a poor substrate. Relative activity compared to glucose 6-phosphate is 0.05%
758932
Lactococcus lactis ssp. lactis
alpha-D-Glc-(1->1)-alpha-D-Man6P + phosphate
-
-
-
?
mannose 6-phosphate + beta-D-glucose 1-phosphate
mannose 6-phosphate is a poor substrate. Relative activity compared to glucose 6-phosphate is 0.05%
758932
Lactococcus lactis ssp. lactis JCM 5805
alpha-D-Glc-(1->1)-alpha-D-Man6P + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 90000, SDS-PAGE
Lactococcus lactis ssp. lactis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Lactococcus lactis ssp. lactis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
50
25°C: about 45% of maximal activity, 50°C: about 80% of maximal activity
Lactococcus lactis ssp. lactis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
activity is retained after incubation at pH 5.0-8.0 at 30°C for 15 min
Lactococcus lactis ssp. lactis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
759
-
glucose 6-phosphate
pH 5.5, 30°C
Lactococcus lactis ssp. lactis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.8
-
synthesis of alpha,alpha-trehalose 6-phosphate
Lactococcus lactis ssp. lactis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4.5
9
activity is retained after incubation at pH 4.5-9.0 at 4°C for 24 h
Lactococcus lactis ssp. lactis
5
8
activity is retained after incubation at pH 5.0-8.0 at 30°C for 15 min
Lactococcus lactis ssp. lactis
Other publictions for EC 2.4.1.216
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
759004
Taguchi
Efficient one-pot enzymatic s ...
Lactococcus lactis subsp. lactis
Carbohydr. Res.
488
107902
2020
-
1
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
758932
Taguchi
Evaluation of acceptor select ...
Lactococcus lactis ssp. lactis, Lactococcus lactis ssp. lactis JCM 5805
Biosci. Biotechnol. Biochem.
81
1512-1519
2017
-
-
1
-
-
-
1
2
-
-
3
-
-
5
-
-
1
-
-
-
-
1
6
1
1
1
1
1
1
1
-
2
-
1
-
-
-
-
1
-
-
-
-
-
1
1
2
-
-
3
-
-
-
-
1
-
-
-
1
6
1
1
1
1
1
1
-
2
-
-
-
-
-
-
-
736176
Shi
Trehalose metabolism in the bl ...
Callinectes sapidus
Gene
536
105-113
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721344
Carvalho
Engineering trehalose synthesi ...
Lactococcus lactis, Lactococcus lactis MG1363
Appl. Environ. Microbiol.
77
4189-4199
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
395984
Andersson U.; Radström
beta-Glucose 1-phosphate-inter ...
Carnobacterium divergens, Carnobacterium maltaromaticum, Carnobacterium sp., Enterococcus faecalis, Enterococcus faecium, Lactiplantibacillus plantarum, Lactobacillus casei ssp. casei, Lactobacillus delbrueckii ssp. delbrueckii, Lactiplantibacillus pentosus, Limosilactobacillus reuteri, Weissella viridescens, Lactococcus lactis, Lactococcus lactis ssp. cremoris, Lactococcus lactis ssp. lactis, Lactococcus plantarum, Lactococcus raffinolactis, Leuconostoc mesenteroides ssp. dextranicum, Oenococcus oeni, Leuconostoc pseudomesenteroides, Leuconostoc weissella, Pediococcus acidilactici, Pediococcus damnosus, Tetragenococcus halophilus, Pediococcus pentosaceus, Vagococcus salmoninarum, Carnobacterium sp. 496
Environ. Microbiol.
4
81-88
2002
-
-
-
-
-
-
-
-
-
-
-
26
-
30
-
-
-
-
-
25
2
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
26
-
-
-
-
-
25
2
-
26
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395985
Andersson
Trehalose-6-phosphate phosphor ...
Lactococcus lactis, Lactococcus lactis IL1403
J. Biol. Chem.
276
42707-42713
2001
-
-
1
-
-
-
-
4
-
-
1
2
-
10
-
-
1
-
-
2
1
-
2
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
1
2
-
-
-
1
-
2
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-