Literature summary for 2.4.1.214 extracted from

Kurz, S.; King, J.G.; Dinglasan, R.R.; Paschinger, K.; Wilson, I.B.
The fucomic potential of mosquitoes: Fucosylated N-glycan epitopes and their cognate fucosyltransferases (2016), Insect Biochem. Mol. Biol., 68, 52-63.

Search for more literature for 2.4.1.214

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris GS115 cells	Anopheles gambiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	about 45% residual activity at 10 mM; about 75% residual activity at 10 mM	Anopheles gambiae
Co2+	about 30% residual activity at 10 mM; about 35% residual activity at 10 mM	Anopheles gambiae
Cu2+	about 28% residual activity at 10 mM; no activity at 10 mM	Anopheles gambiae
EDTA	about 60% residual activity at 10 mM; about 8% residual activity at 10 mM	Anopheles gambiae
Mg2+	about 45% residual activity at 10 mM; about 70% residual activity at 10 mM	Anopheles gambiae
Ni2+	about 38% residual activity at 10 mM; about 5% residual activity at 10 mM	Anopheles gambiae
Zn2+	about 10% residual activity at 10 mM; about 1% residual activity at 10 mM	Anopheles gambiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Anopheles gambiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	x * 46000, SDS-PAGE	Anopheles gambiae
61000	-	x * 61000, SDS-PAGE	Anopheles gambiae

Organism

Organism	UniProt	Comment	Textmining
Anopheles gambiae	Q7QBF7	-	-
Anopheles gambiae	Q7QF91	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Affi-Gel Blue Sepharose column chromatography	Anopheles gambiae

Source Tissue

Source Tissue	Comment	Organism	Textmining
adult	-	Anopheles gambiae	-
larva	-	Anopheles gambiae	-

Subunits

Subunits	Comment	Organism
?	x * 46000, SDS-PAGE	Anopheles gambiae
?	x * 61000, SDS-PAGE	Anopheles gambiae

Synonyms

Synonyms	Comment	Organism
FucTA	-	Anopheles gambiae
FucTC	-	Anopheles gambiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	-	Anopheles gambiae
30	-	-	Anopheles gambiae

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	40	more than 50% activity between 10 and 40°C	Anopheles gambiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	6.5	-	Anopheles gambiae
7	7.5	-	Anopheles gambiae

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	7	more than 50% activity between pH 5.0 and 7.0	Anopheles gambiae
6.5	8	more than 50% activity between pH 6.5 and 8.0	Anopheles gambiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)