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Literature summary for 2.4.1.214 extracted from

  • Koetzler, M.P.; Blank, S.; Behnken, H.N.; Alpers, D.; Bantleon, F.I.; Spillner, E.; Meyer, B.
    Formation of the immunogenic alpha1,3-fucose epitope: elucidation of substrate specificity and of enzyme mechanism of core fucosyltransferase A (2012), Insect Biochem. Mol. Biol., 42, 116-125.
    View publication on PubMed
Search for more literature for 2.4.1.214

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Spodoptera frugiperda Sf9 cells Apis mellifera

Inhibitors

Inhibitors Comment Organism Structure
GDP strong product inhibition Apis mellifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
 GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc at 25°C, pH not specified in the publication Apis mellifera
1
-
 GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc at 25°C, pH not specified in the publication Apis mellifera

Organism

Organism UniProt Comment Textmining
Apis mellifera
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Apis mellifera

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Apis mellifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + (1->4)-beta-D-galactosyl-N-acetyl-D-glucosaminyl-L-Asn
-
 Apis mellifera GDP + (1->4)-beta-D-galactosyl-[alpha-(1->3)-L-fucosyl]-N-acetyl-D-glucosaminyl-L-Asn
-
 ?
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
 Apis mellifera GDP + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)]GlcNAc
-
 ?
GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc
-
 Apis mellifera GDP + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc
-
 ?

Synonyms

Synonyms Comment Organism
alpha1,3-fucosyltransferase
-
 Apis mellifera
fucosyltransferase A
-
 Apis mellifera
FucTA
-
 Apis mellifera

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.097
-
 GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc at 25°C, pH not specified in the publication Apis mellifera
0.11
-
 GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc at 25°C, pH not specified in the publication Apis mellifera