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Literature summary for 2.4.1.214 extracted from

  • Lin, S.W.; Yuan, T.M.; Li, J.R.; Lin, C.H.
    Carboxyl terminus of Helicobacter pylori alpha1,3-fucosyltransferase determines the structure and stability (2006), Biochemistry, 45, 8108-8116.
    View publication on PubMed
Search for more literature for 2.4.1.214

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli DH5alpha or BL21 Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
additional information structural analysis of the FT of Helicobacter pylori by systematic C-terminal truncation reveals that up to 80 residues, including the region rich in hydrophobic and positively charged residues (434-478) and 5 of the 10 tandem repeats of 7 amino acids each (399-433) can be removed without significant change in structure and catalysis, half of the heptad repeats required to maintain both the secondary and native quaternary structures, systematic deletion of the C-terminus considerably improves the solubility of the protein, truncated forms are highly specific for type 2 sugar substrates that contain the Galbeta-1,4-GlcNAc structure, type 1 substrate Galbeta-1,3-GlcNAc is not accepted Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0683
-
 GDP-fucose deletion mutant FTdelta23 with 23 residues deleted from the C-terminus Helicobacter pylori
0.07864
-
 GDP-fucose deletion mutant FTdelta66 with 66 residues deleted from the C-terminus Helicobacter pylori
0.07967
-
 GDP-fucose deletion mutant FTdelta38 with 38 residues deleted from the C-terminus Helicobacter pylori
0.08831
-
 GDP-fucose deletion mutant FTdelta45 with 45 residues deleted from the C-terminus Helicobacter pylori
0.09356
-
 GDP-fucose deletion mutant FTdelta28 with 28 residues deleted from the C-terminus Helicobacter pylori
0.09864
-
 GDP-fucose deletion mutant FTdelta80 with 80 residues deleted from the C-terminus Helicobacter pylori
0.228
-
 GDP-fucose deletion mutant FTdelta115 with 115 residues deleted from the C-terminus Helicobacter pylori
0.7
-
 LacNAc deletion mutant FTdelta38 with 38 residues deleted from the C-terminus Helicobacter pylori
0.71
-
 LacNAc deletion mutant FTdelta45 with 45 residues deleted from the C-terminus Helicobacter pylori
0.71
-
 LacNAc deletion mutant FTdelta66 with 66 residues deleted from the C-terminus Helicobacter pylori
0.78
-
 LacNAc deletion mutant FTdelta23 with 23 residues deleted from the C-terminus Helicobacter pylori
0.78
-
 LacNAc deletion mutant FTdelta28 with 28 residues deleted from the C-terminus Helicobacter pylori
0.89
-
 LacNAc deletion mutant FTdelta80 with 80 residues deleted from the C-terminus Helicobacter pylori
8.92
-
 LacNAc deletion mutant FTdelta115 with 115 residues deleted from the C-terminus Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori O30511
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.38
-
 deletion mutant FTdelta115 with 115 residues deleted from the C-terminus, substrate LacNAc Helicobacter pylori
2.52
-
 deletion mutant FTdelta23 with 23 residues deleted from the C-terminus, substrate LacNAc Helicobacter pylori
4.84
-
 deletion mutant FTdelta28 with 28 residues deleted from the C-terminus, substrate LacNAc Helicobacter pylori
5.04
-
 deletion mutant FTdelta38 with 38 residues deleted from the C-terminus, substrate LacNAc Helicobacter pylori
5.2
-
 deletion mutant FTdelta80 with 80 residues deleted from the C-terminus, substrate LacNAc Helicobacter pylori
5.72
-
 deletion mutant FTdelta45 with 45 residues deleted from the C-terminus, substrate LacNAc Helicobacter pylori
5.82
-
 deletion mutant FTdelta66 with 66 residues deleted from the C-terminus, substrate LacNAc Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + N-acetyllactosamine
-
 Helicobacter pylori GDP + 3-fucosyl-N-acetyllactosamine
-
 ?

Synonyms

Synonyms Comment Organism
alpha1,3-fucosyltransferase
-
 Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 glycosyltransferase assay Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 glycosyltransferase assay Helicobacter pylori