Cloned (Comment) | Organism |
---|---|
cDNA cloning of Fuc-T C3 from mRNA, recombinant enzyme expressed in Sf21 insect cells using baculovirus vector | Vigna radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
GDP-fucose | - |
Vigna radiata | |
0.13 | - |
GnGnF6-peptide | - |
Vigna radiata | |
0.19 | - |
GnGn-peptide | - |
Vigna radiata | |
0.23 | - |
M5Gn-Asn | - |
Vigna radiata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Vigna radiata | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, major isoform, SDS-PAGE | Vigna radiata |
56000 | - |
x * 56000, represents the same enzyme as the band with 54kDA, SDS-PAGE | Vigna radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | Vigna radiata | - |
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of Fuc-T C3, using Triton X-100 extraction and column chromatography on DE52 cellulose, Affi-Gel blue, S-Sepharose, GnGn-Sepharose and GDP-hexanolamine-Sepharose | Vigna radiata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43.35 | - |
- |
Vigna radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + dabsylated-GalGal-peptide | 0.7% relative activity with recombinant enzyme | Vigna radiata | GDP + alpha1-3-fucosylated peptide | - |
? | |
GDP-fucose + dabsylated-GalGn-peptide | 50% relative activity with recombinant enzyme | Vigna radiata | GDP + alpha1-3-fucosylated peptide | - |
? | |
GDP-fucose + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAcbeta1-Asn | - |
Vigna radiata | GDP + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)(Fucalpha1-3)GlcNAcbeta1-Asn | - |
? | |
GDP-fucose + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAcbeta1-Asn | - |
Vigna radiata | GDP + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-Asn | - |
? | |
GDP-fucose + GlcNAcbeta1-2-Manalpha1-3[Manalpha1-3(Manalpha1-6)Manalpha1-6]Manbeta1-4-GlcNAcbeta1-4GlcNAcbeta1-Asn | - |
Vigna radiata | GDP + GlcNAcbeta1-2-Manalpha1-3[Manalpha1-3(Manalpha1-6)Manalpha1-6]Manbeta1-4-GlcNAcbeta1-4 (Fucalpha1-3)GlcNAcbeta1-Asn | - |
? | |
GDP-fucose + GnGn-peptide | 100% relative activity with recombinant enzyme | Vigna radiata | GDP + alpha1-3-fucosylated peptide | - |
? | |
GDP-fucose + GnGnF6-peptide | 87% relative activity with recombinant enzyme | Vigna radiata | GDP + alpha1-3-fucosylated GnGnF6-peptide | - |
? | |
GDP-fucose + M5Gn-Asn | 71% relative activity with recombinant enzyme | Vigna radiata | GDP + alpha1-3-fucosylated M5Gn-Asn | - |
? | |
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Vigna radiata | GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, major isoform, SDS-PAGE | Vigna radiata |
? | x * 56000, represents the same enzyme as the band with 54kDA, SDS-PAGE | Vigna radiata |