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Literature summary for 2.4.1.214 extracted from

  • Leiter, H.; Mucha, J.; Staudacher, E.; Grimm, R.; Glossl, J.; Altmann, F.
    Purification, cDNA cloning, and expression of GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase from mung beans (1999), J. Biol. Chem., 274, 21830-21839.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA cloning of Fuc-T C3 from mRNA, recombinant enzyme expressed in Sf21 insect cells using baculovirus vector Vigna radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
GDP-fucose
-
Vigna radiata
0.13
-
GnGnF6-peptide
-
Vigna radiata
0.19
-
GnGn-peptide
-
Vigna radiata
0.23
-
M5Gn-Asn
-
Vigna radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Vigna radiata
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000, major isoform, SDS-PAGE Vigna radiata
56000
-
x * 56000, represents the same enzyme as the band with 54kDA, SDS-PAGE Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine Vigna radiata
-
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
?

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of Fuc-T C3, using Triton X-100 extraction and column chromatography on DE52 cellulose, Affi-Gel blue, S-Sepharose, GnGn-Sepharose and GDP-hexanolamine-Sepharose Vigna radiata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43.35
-
-
Vigna radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + dabsylated-GalGal-peptide 0.7% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated peptide
-
?
GDP-fucose + dabsylated-GalGn-peptide 50% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated peptide
-
?
GDP-fucose + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAcbeta1-Asn
-
Vigna radiata GDP + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)(Fucalpha1-3)GlcNAcbeta1-Asn
-
?
GDP-fucose + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAcbeta1-Asn
-
Vigna radiata GDP + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-Asn
-
?
GDP-fucose + GlcNAcbeta1-2-Manalpha1-3[Manalpha1-3(Manalpha1-6)Manalpha1-6]Manbeta1-4-GlcNAcbeta1-4GlcNAcbeta1-Asn
-
Vigna radiata GDP + GlcNAcbeta1-2-Manalpha1-3[Manalpha1-3(Manalpha1-6)Manalpha1-6]Manbeta1-4-GlcNAcbeta1-4 (Fucalpha1-3)GlcNAcbeta1-Asn
-
?
GDP-fucose + GnGn-peptide 100% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated peptide
-
?
GDP-fucose + GnGnF6-peptide 87% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated GnGnF6-peptide
-
?
GDP-fucose + M5Gn-Asn 71% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated M5Gn-Asn
-
?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
Vigna radiata GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
?

Subunits

Subunits Comment Organism
? x * 54000, major isoform, SDS-PAGE Vigna radiata
? x * 56000, represents the same enzyme as the band with 54kDA, SDS-PAGE Vigna radiata