Cloned (Comment) | Organism |
---|---|
three genes FucTA, FucTB and FucTC encode proteins similar to alpha1-3-fucosyltransferases, expression in Pichia pastoris | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
dansyl-GnGn | FucTA gene | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
FucTB gene, MALDI peptide mapping and Western blotting | Arabidopsis thaliana |
56210 | - |
FucTA gene, amino acid sequence analysis | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | Arabidopsis thaliana | - |
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? | |
additional information | Arabidopsis thaliana | the core alpha1-3-fucosyltransferases are involved in the synthesis of glycans specific to plants and invertebrates which are known to be immunogenic and allergenic | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + dabsyl-GnGn-peptide | FucTA gene, dabsylated glycopeptide derived from fibrin carrying a GnGn oligosaccharide | Arabidopsis thaliana | GDP + alpha1-3-fucosylated dabsyl-GnGn-peptide | - |
? | |
GDP-fucose + dansyl-GnGn | FucTA gene, dansylated glycopeptide derived from IgG, 40% relative conversion | Arabidopsis thaliana | GDP + alpha1-3-fucosylated glycopeptide | - |
? | |
GDP-fucose + dansylated mono-beta1,3-galactosylated glycopeptide | FucTA gene | Arabidopsis thaliana | GDP + alpha1-3-fucosylated glycopeptide | - |
? | |
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Arabidopsis thaliana | GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? | |
additional information | FucTA gene, amongst the dansyl glycopeptides tested, only those with one free non-reducing terminal N-acetyl-glucosamine residue act as substrates: specifically GnGn lacking one GlcNAc, GnGn substituted with one galactose residue or GnGn substituted with one Lewis-a epitope | Arabidopsis thaliana | ? | - |
? | |
additional information | the core alpha1-3-fucosyltransferases are involved in the synthesis of glycans specific to plants and invertebrates which are known to be immunogenic and allergenic | Arabidopsis thaliana | ? | - |
? |