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Literature summary for 2.4.1.214 extracted from

  • Wilson, I.B.H.; Rendic, D.; Freilinger, A.; Dumic, J.; Altmann, F.; Mucha, J.; Müller, S.; Hauser, M.T.
    Cloning and expression of alpha1,3-fucosyltransferase homologues from Arabidopsis thaliana (2001), Biochim. Biophys. Acta, 1527, 88-96.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
three genes FucTA, FucTB and FucTC encode proteins similar to alpha1-3-fucosyltransferases, expression in Pichia pastoris Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
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dansyl-GnGn FucTA gene Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
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FucTB gene, MALDI peptide mapping and Western blotting Arabidopsis thaliana
56210
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FucTA gene, amino acid sequence analysis Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine Arabidopsis thaliana
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GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
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?
additional information Arabidopsis thaliana the core alpha1-3-fucosyltransferases are involved in the synthesis of glycans specific to plants and invertebrates which are known to be immunogenic and allergenic ?
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?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-fucose + dabsyl-GnGn-peptide FucTA gene, dabsylated glycopeptide derived from fibrin carrying a GnGn oligosaccharide Arabidopsis thaliana GDP + alpha1-3-fucosylated dabsyl-GnGn-peptide
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?
GDP-fucose + dansyl-GnGn FucTA gene, dansylated glycopeptide derived from IgG, 40% relative conversion Arabidopsis thaliana GDP + alpha1-3-fucosylated glycopeptide
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?
GDP-fucose + dansylated mono-beta1,3-galactosylated glycopeptide FucTA gene Arabidopsis thaliana GDP + alpha1-3-fucosylated glycopeptide
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?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
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Arabidopsis thaliana GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
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?
additional information FucTA gene, amongst the dansyl glycopeptides tested, only those with one free non-reducing terminal N-acetyl-glucosamine residue act as substrates: specifically GnGn lacking one GlcNAc, GnGn substituted with one galactose residue or GnGn substituted with one Lewis-a epitope Arabidopsis thaliana ?
-
?
additional information the core alpha1-3-fucosyltransferases are involved in the synthesis of glycans specific to plants and invertebrates which are known to be immunogenic and allergenic Arabidopsis thaliana ?
-
?