Literature summary for 2.4.1.212 extracted from

Lai, Z.; Teo, C.
Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture (2019), Malays. J. Microbiol., 15, 575-582 .

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Application

Application	Comment	Organism
synthesis	the hasA gene is cloned and expressed in Escherichia coli BL21 and the use this recombinant Escherichia coli strain BL21 is used as host in order to explore the biosynthetic capabilities for hyaluronan production (concentration and molecular weight) in batch fermentation using shake flask culture. High hyaluronan production (0.115 g/l) is achieved in nutrient rich media with 50 g/l glucose concentration. With addition of 1.0 mM isopropyl beta-D-1-thiogalactopyranoside, the highest hyaluronan production (0.532 g/l) and molecular weight at 34600 Da is achieved which is around fivefold higher compared to without isopropyl beta-D-1-thiogalactopyranoside	Streptococcus equi subsp. zooepidemicus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Streptococcus equi subsp. zooepidemicus

Organism

Organism	UniProt	Comment	Textmining
Streptococcus equi subsp. zooepidemicus	Q84GC8	-	-

Synonyms

Synonyms	Comment	Organism
hasA	-	Streptococcus equi subsp. zooepidemicus

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Release 2023.1 (January 2023)