Literature summary for 2.4.1.212 extracted from

Zhang, L.; Huang, H.; Wang, H.; Chen, J.; Du, G.; Kang, Z.
Rapid evolution of hyaluronan synthase to improve hyaluronan production and molecular mass in Bacillus subtilis (2016), Biotechnol. Lett., 38, 2103-2108 .

Search for more literature for 2.4.1.212

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Bacillus subtilis	Streptococcus equi subsp. zooepidemicus

Protein Variants

Protein Variants	Comment	Organism
N196I/L197R/T202S/D203H/C226F/S231D/V232F/E236Q/S256N/C262S/K294T/N297H/N300K/F303C	the enzyme is engineered to increase hyaluronan production and molecular mass through structural alteration of multiple regions. As compared with other variants and wild-type enzyme, the V5 variant generates a higher hyaluronan titer (2.24 g/l) and molecular weight value (1360000 Da). Following overexpression of the genes tuaD and glmU in Bacillus subtilis V5, hyaluronan production and molecular weight increases further to 2.81 g/l and 2430000 Da, respectively. The results provide a new strategy for producing hyaluronan with higher titers and molecular mass values that could be extended to other polysaccharides, such as heparosan and chondroitin, produced in Bacillus subtilis	Streptococcus equi subsp. zooepidemicus

Organism

Organism	UniProt	Comment	Textmining
Streptococcus equi subsp. zooepidemicus	Q84GC8	-	-

Synonyms

Synonyms	Comment	Organism
HAS	-	Streptococcus equi subsp. zooepidemicus
hasA	-	Streptococcus equi subsp. zooepidemicus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)