Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Streptococcus equi subsp. zooepidemicus |
Protein Variants | Comment | Organism |
---|---|---|
N196I/L197R/T202S/D203H/C226F/S231D/V232F/E236Q/S256N/C262S/K294T/N297H/N300K/F303C | the enzyme is engineered to increase hyaluronan production and molecular mass through structural alteration of multiple regions. As compared with other variants and wild-type enzyme, the V5 variant generates a higher hyaluronan titer (2.24 g/l) and molecular weight value (1360000 Da). Following overexpression of the genes tuaD and glmU in Bacillus subtilis V5, hyaluronan production and molecular weight increases further to 2.81 g/l and 2430000 Da, respectively. The results provide a new strategy for producing hyaluronan with higher titers and molecular mass values that could be extended to other polysaccharides, such as heparosan and chondroitin, produced in Bacillus subtilis | Streptococcus equi subsp. zooepidemicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus equi subsp. zooepidemicus | Q84GC8 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
HAS | - |
Streptococcus equi subsp. zooepidemicus |
hasA | - |
Streptococcus equi subsp. zooepidemicus |