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Literature summary for 2.4.1.212 extracted from

  • Takabe, P.; Bart, G.; Ropponen, A.; Rilla, K.; Tammi, M.; Tammi, R.; Pasonen-Seppaenen, S.
    Hyaluronan synthase 3 (HAS3) overexpression downregulates MV3 melanoma cell proliferation, migration and adhesion (2015), Exp. Cell Res., 337, 1-15.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
isozyme HAS3 inducible overexpression in MV3/C8161 cells. EGFP-HAS3 overexpression rapidly induced pericellular hyaluronan coat formation and plasma membrane protrusions, induction by doxycycline Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information isozyme HAS3 overexpression expands the cell surface hyaluronan coat and decreases melanoma cell adhesion, migration and proliferation by cell cycle arrest at G1/G0. Melanoma cell migration is restored by removal of cell surface hyaluronan by Streptomyces hyaluronidase and by receptor blocking with hyaluronan oligosaccharides, while the effect on cell proliferation is receptor independent. Overexpression of isozyme HAS3 decreases ERK1/2 phosphorylation suggesting that inhibition of MAP-kinase signaling is responsible for the suppressive effects on the malignant phenotype of MV3 melanoma cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Homo sapiens 5794
-
plasma membrane
-
 Homo sapiens 5886
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
Has3
-
 Homo sapiens
hyaluronan synthase 3
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens doxycycline treatment causes a strong upregulation of HAS3 expression. A distinct EGFP-HAS3 signal is observed already 2 h after starting the doxycycline induction, localized mainly at the plasma membrane and in the Golgi area. After 24 h induction EGFP-HAS3 signal has become more intense, localizing strongly at the Golgi area and at the plasma membrane up

General Information

General Information Comment Organism
malfunction isozyme HAS3 overexpression downregulates MV3 melanoma cell proliferation, migration and adhesion. Overexpression of isozyme HAS3 decreases cell proliferation, directional and random cell migration, and promotes cell cycle arrest at G1/G0 and decreases ERK1/2 phosphorylation suggesting that inhibition of MAP-kinase signaling is responsible for the suppressive effects on the malignant phenotype of MV3 melanoma cells. EGFP-HAS3 overexpression downregulates several signaling pathways in MV3 melanoma cells Homo sapiens
physiological function the enzyme is involved in synthesis of hyaluronan that may have anti-cancer like effects in melanoma progression Homo sapiens