Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bifidobacterium bifidum JCM 1254 |
Protein Variants | Comment | Organism |
---|---|---|
D108N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D160N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D182N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D232N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D260N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D280N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D29N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D313N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D331N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D344N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D348N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D35N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D373N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D490N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D491N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
D561N | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
E249Q | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
E259Q | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
E319Q | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
E356Q | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
E377Q | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
E475Q | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
E568Q | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
E691Q | site-directed mutagenesis | Bifidobacterium bifidum JCM 1254 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum JCM 1254 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant proteins by Ni-NTA spin column chromatography | Bifidobacterium bifidum JCM 1254 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
D313N, no activity, D313 is probably the catalytic proton donor site | Bifidobacterium bifidum JCM 1254 |
0.06 | - |
E356Q | Bifidobacterium bifidum JCM 1254 |
0.17 | - |
D29N | Bifidobacterium bifidum JCM 1254 |
0.22 | - |
D160N | Bifidobacterium bifidum JCM 1254 |
0.31 | - |
E319Q | Bifidobacterium bifidum JCM 1254 |
0.53 | - |
E568Q | Bifidobacterium bifidum JCM 1254 |
0.72 | - |
D232N | Bifidobacterium bifidum JCM 1254 |
0.9 | - |
D182N | Bifidobacterium bifidum JCM 1254 |
1.68 | - |
E259Q | Bifidobacterium bifidum JCM 1254 |
2.85 | - |
D280N | Bifidobacterium bifidum JCM 1254 |
3.61 | - |
D35N | Bifidobacterium bifidum JCM 1254 |
3.93 | - |
E377Q | Bifidobacterium bifidum JCM 1254 |
4.79 | - |
E475Q | Bifidobacterium bifidum JCM 1254 |
5.41 | - |
D491N | Bifidobacterium bifidum JCM 1254 |
5.71 | - |
D348N | Bifidobacterium bifidum JCM 1254 |
5.73 | - |
E249Q | Bifidobacterium bifidum JCM 1254 |
5.84 | - |
E691Q | Bifidobacterium bifidum JCM 1254 |
5.9 | - |
D561N | Bifidobacterium bifidum JCM 1254 |
6.09 | - |
D331N | Bifidobacterium bifidum JCM 1254 |
6.71 | - |
D490N | Bifidobacterium bifidum JCM 1254 |
7.26 | - |
D108N | Bifidobacterium bifidum JCM 1254 |
7.32 | - |
D344N | Bifidobacterium bifidum JCM 1254 |
7.98 | - |
D373N | Bifidobacterium bifidum JCM 1254 |
9.2 | - |
D260N | Bifidobacterium bifidum JCM 1254 |
10 | - |
LNBP2 | Bifidobacterium bifidum JCM 1254 |
10.5 | - |
LNBP1, all mutants display decreased activities, D313N has no activity | Bifidobacterium bifidum JCM 1254 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate | - |
Bifidobacterium bifidum JCM 1254 | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Bifidobacterium bifidum JCM 1254 | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lacto-N-biose phosphorylase | - |
Bifidobacterium bifidum JCM 1254 |
lnba1 | isoform LNBP1 | Bifidobacterium bifidum JCM 1254 |
lnba2 | isoform LNBP2 | Bifidobacterium bifidum JCM 1254 |
LNBP | - |
Bifidobacterium bifidum JCM 1254 |