Any feedback?
Literature summary for 2.4.1.210 extracted from
- Preparation and properties of immobilized pummelo limonoid glucosyltransferase (2002), Proc. Biochem., 38, 809-814.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | exploration of the possibility of using the enzyme in a bioreactor for debittering citrus juice | Citrus maxima |
General Stability
| General Stability | Organism |
|---|---|
| immobilized LGTase retains more than 80% activity up to 15 cycles on cellulose carbonate, nine cycles on chitosan and six cycles on DEAE-Toyopearl | Citrus maxima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
limonin | pH 7.8, 37°C, soluble enzyme | Citrus maxima |  |
| 0.0813 | - |
limonin | pH 7.8, 37°C, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde | Citrus maxima | |
| 0.091 | - |
limonin | pH 7.8, 37°C, enzyme immobilized covalently on cellulose carbonate | Citrus maxima | |
| 0.1266 | - |
limonin | pH 7.8, 37°C, enzyme immobilized ionically on DEAE-Toyopearl | Citrus maxima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus maxima | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Citrus maxima | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + limonin | - |
Citrus maxima | glucosyl-limonin + UDP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LGTase | - |
Citrus maxima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
soluble enzyme and enzyme immobilized ionically on DEAE-Toyopearl (DE-Tp-LGTase) | Citrus maxima |
| 40 | - |
enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde (Chito-GA-LGTase) and enzyme immobilized covalently on cellulose carbonate (Ce-Ca-LGTase) | Citrus maxima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 20°C: about 55% of maximal activity, 70°C: about 30% of maximal activity | Citrus maxima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 7.5, 3 h, Ce-Ca-LGTase retains 65.3%, free LGTase retains 19.2% | Citrus maxima |
| 60 | - |
pH 7.5, 30 min, free enzyme retains 55% of the activity, enzyme immobilized ionically on DEAE-Toyopearl retains 80% of the activity, enzyme immobilized covalently on cellulose carbonate retains 45% of the activity, enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde retains 40% of the activity | Citrus maxima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
enzyme immobilized covalently on chitosan cross-linked with gluteraldehyde (Chito-GA-LGTase) | Citrus maxima |
| 7.8 | - |
soluble enzyme or enzyme immobilized covalently on cellulose carbonate (Ce-Ca-LGTase) | Citrus maxima |
| 8.2 | - |
enzyme immobilized ionically on DEAE-Toyopearl (DE-Tp-LGTase) | Citrus maxima |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
