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Literature summary for 2.4.1.21 extracted from

  • Barchiesi, J.; Velazquez, M.B.; Palopoli, N.; Iglesias, A.A.; Gomez-Casati, D.F.; Ballicora, M.A.; Busi, M.V.
    Starch synthesis in Ostreococcus tauri The starch-binding domains of starch synthase III-B are essential for catalytic activity (2018), Front. Plant Sci., 9, 1541 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
isoform SSIII-B is expressed in Escherichia coli BL21(DE3) pLysS cells Ostreococcus tauri

Protein Variants

Protein Variants Comment Organism
F138A the mutant shows reduced activity compared to the wild type enzyme Ostreococcus tauri
W122A/Y124A the mutant shows reduced activity compared to the wild type enzyme Ostreococcus tauri
W279A the mutant shows reduced activity compared to the wild type enzyme Ostreococcus tauri
W304A the mutant shows reduced activity compared to the wild type enzyme Ostreococcus tauri
Y147A the mutant shows reduced activity compared to the wild type enzyme Ostreococcus tauri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n Ostreococcus tauri
-
 ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Ostreococcus tauri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography Ostreococcus tauri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + potato amylopectin amylopectin is a high affinity substrate Ostreococcus tauri ADP + ?
-
 ?
ADP-alpha-D-glucose + potato amylose low activity Ostreococcus tauri ADP + ?
-
 ?
ADP-alpha-D-glucose + potato starch low activity Ostreococcus tauri ADP + ?
-
 ?
ADP-alpha-D-glucose + rabbit liver glycogen glycogen is a high affinity substrate Ostreococcus tauri ADP + ?
-
 ?
ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n
-
 Ostreococcus tauri ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Subunits

Subunits Comment Organism
? x * 103200, calculated from amino acid sequence Ostreococcus tauri

Synonyms

Synonyms Comment Organism
SSIII-B isoform Ostreococcus tauri
starch synthase III-B
-
 Ostreococcus tauri