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Literature summary for 2.4.1.21 extracted from

  • Barchiesi, J.; Hedin, N.; Iglesias, A.A.; Gomez-Casati, D.F.; Ballicora, M.A.; Busi, M.V.
    Identification of a novel starch synthase III from the picoalgae Ostreococcus tauri (2017), Biochimie, 133, 37-44 .
    View publication on PubMed
Search for more literature for 2.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Ostreococcus tauri

Protein Variants

Protein Variants Comment Organism
E352A the mutation renders the enzyme about 146 and 14fold less active regards ADP-alpha-D-glucose and rabbit muscle glycogen, respectively Ostreococcus tauri
K275A the mutation renders the enzyme about 327 and 74fold less active regards ADP-alpha-D-glucose and rabbit muscle glycogen, respectively Ostreococcus tauri
R270A the mutation renders the enzyme about 301 and 52fold less active regards ADP-alpha-D-glucose and rabbit muscle glycogen, respectively Ostreococcus tauri

Organism

Organism UniProt Comment Textmining
Ostreococcus tauri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography Ostreococcus tauri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + amylopectin
-
 Ostreococcus tauri ADP + ?
-
 ?
ADP-alpha-D-glucose + rabbit muscle glycogen
-
 Ostreococcus tauri ADP + ?
-
 ?
additional information the enzyme is not active when UDP-alpha-D-glucose is used as the glucosyl donor substrate Ostreococcus tauri ?
-
-

Subunits

Subunits Comment Organism
? x * 56800, SDS-PAGE Ostreococcus tauri

Synonyms

Synonyms Comment Organism
glycogen/starch synthase
-
 Ostreococcus tauri
SSIII-C isoform Ostreococcus tauri
starch synthase III
-
 Ostreococcus tauri