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Literature summary for 2.4.1.21 extracted from
- Biosynthesis of bacterial glycogen. Purification and properties of the Escherichia coli B ADPglucose:1,4-alpha-D-glucan 4-alpha-glucosyltransferase (1976), Biochemistry, 15, 849-856.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucono-1,5-lactone | inhibition of glycogen synthase I and II | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | E. coli B glycogen: 0.274 mg/ml, rabbit liver glycogen: 0.298 mg/ml, soluble potato amylose: 0.833 mg/ml | Escherichia coli | |
| 0.035 | - |
ADPglucose | glycogen synthases I and II | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
2 * 49000, SDS-PAGE | Escherichia coli |
| 92700 | - |
sucrose density gradient centrifugation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isozymes, DEAE-cellulose, 4-aminobutyl-Sepharose, Bio-Gel P200 | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 494 | - |
glycogen synthase I | Escherichia coli |
| 505 | - |
glcogen synthase II | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C, at least 1 year | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + alpha-1,4-polyglucan | citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen | Escherichia coli | ADP + alpha-1,4-polyglucan | - |
r | |
| ADP-glucose + alpha-1,4-polyglucan | glucosyl acceptors: maltose, maltotriose, maltotetraose and maltoheptaose | Escherichia coli | ADP + alpha-1,4-polyglucan | - |
r | |
| ADP-glucose + alpha-1,4-polyglucan | glucosyl acceptor: amylose | Escherichia coli | ADP + alpha-1,4-polyglucan | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 49000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | - |
Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
D-glucono-1,5-lactone | - |
Escherichia coli | |
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