Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,2-dioleoylphosphatidylethanolamine | maximal activity at 4.5% | Acholeplasma laidlawii | |
1,2-dioleoylphosphatidylglycerol | - |
Acholeplasma laidlawii | |
1,2-dioleoylphosphatidylserine | 33% of activation with 1,2-dioleoylphosphatidylglycerol | Acholeplasma laidlawii | |
1,3-dioleoylglycerol | 11.5 mol%, 5fold activation | Acholeplasma laidlawii | |
diphosphatidylglycerol | from bovine, 50% of activation with 1,2-dioleoylphosphatidylglycerol | Acholeplasma laidlawii | |
phosphate | 40 mM, approx. 8fold activation | Acholeplasma laidlawii | |
phosphatidylglycerol | enzyme needs a polar activator lipid, preferably phosphatidylglycerol at concentrations of 25-30 mol% in zwitterionic mixed CHAPS bilayer-like aggregates | Acholeplasma laidlawii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol | - |
Acholeplasma laidlawii | |
1,2-diacyl-3-O-[6-O-acyl(alpha-D-glucopyranosyl)]-sn-glycerol | - |
Acholeplasma laidlawii | |
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-1,2-O-(6-O-acyl-alpha-D-glucopyranosyl)]-sn-glycerol | - |
Acholeplasma laidlawii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
UDP-glucose | - |
Acholeplasma laidlawii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acholeplasma laidlawii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
solubilization, SP-Sepharose, hydroxyapatite, Green A agarose | Acholeplasma laidlawii |
Renatured (Comment) | Organism |
---|---|
enzyme can be reconstituted in liposome bilayers by the dialyse techique | Acholeplasma laidlawii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.05 | - |
- |
Acholeplasma laidlawii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol | no activity if the sugar is in beta-position | Acholeplasma laidlawii | UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol | - |
? | |
UDP-glucose + 1,2-diheptadecanoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol | best substrate | Acholeplasma laidlawii | UDP + 1,2-diheptadecanoyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol | 73% of activity with monoglucosyldioleoylglycerol | ? | |
UDP-glucose + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol | - |
Acholeplasma laidlawii | UDP + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol | - |
? |