Cloned (Comment) | Organism |
---|---|
11 members of the XTH gene family are cloned from Populus tomentosa, sequence comparisons and phylogenetic analysis and tree, recombinant expression of PtoXTH27 in Pichia pastoris strain GS115, sugar and osmotolerance analysis of yeast transformants | Populus tomentosa |
11 members of the XTH gene family are cloned from Populus tomentosa, sequence comparisons and phylogenetic analysis and tree, recombinant expression of PtoXTH34 in Pichia pastoris strain GS115, sugar and osmotolerance analysis of yeast transformants | Populus tomentosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Populus tomentosa | 5618 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tomentosa | A0A2L0E8U4 | - |
- |
Populus tomentosa | A0A2L0E8V5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity of isozymes PtoXTH27 and PtoXTH34, PtoXTH34 has a higher affinity for XXXG. The receptor substrate of XTH in plants may be XXXG, or xyloglucan oligosaccharides with different chain lengths or structures, such as XLLG and XXXGXXXG. The different affinities of PtoXTH34 and PtoXTH27 for XXXG may be due to the diversity of receptor substrates in plants. Additionally, PtoXTH27 may have other receptor substrates with higher affinity | Populus tomentosa | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 33930, PtoXTH27, sequence calculation | Populus tomentosa |
? | x * 34180, PtoXTH34, sequence calculation | Populus tomentosa |
More | comparison with 10 other XTH isozymes from Populus tomentosa, overview | Populus tomentosa |
Synonyms | Comment | Organism |
---|---|---|
PtoXTH27 | - |
Populus tomentosa |
PtoXTH34 | - |
Populus tomentosa |
XET | - |
Populus tomentosa |
XTH | - |
Populus tomentosa |
xyloglucan endotransglucosylase | - |
Populus tomentosa |
xyloglucan endotransglycosylase/hydrolase | - |
Populus tomentosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Populus tomentosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Populus tomentosa |
6 | - |
- |
Populus tomentosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Populus tomentosa | comparison with 10 other XTH genes from Populus tomentosa, overview | - |
additional information |
Populus tomentosa | PtoXTH27, sequence calculation | - |
8.19 |
Populus tomentosa | PtoXTH34, sequence calculation | - |
8.47 |
General Information | Comment | Organism |
---|---|---|
evolution | xyloglucan endotransglycosylase/hydrolase (XTH) belongs to the GH16 subfamily of the glycoside hydrolases of carbohydrate active enzymes. 11 members of the XTH gene family are cloned from Populus tomentosa. A bioinformatics analysis reveals that the 11 PtoXTHs can be classified into three groups. Biochemical analyses of purified PtoXTHs demonstrate that only isozymes PtoXTH27 and PtoXTH34 have detectable xyloglucan endotransglucosylase (XET) activity, while the others do not exhibit XET or XEH activity. PtoXTH34 has a higher affinity for the receptor substrate implying that PtoXTH34 and PtoXTH27 in plants have different substrate structure specificity. Members of the XTH gene family may have one or two enzymatic activities: one is xyloglucan endohydrolase (XEH) activity, which specifically hydrolyzes xyloglucan beta-1,4 glycosidic bonds, and the other is xyloglucan endotransglucosylase (XET) activity, which refers to the transfer of xyloglucan fragments between xyloglucan molecules | Populus tomentosa |
additional information | structural modeling and analysis of PtoXTH proteins | Populus tomentosa |
physiological function | Populus xyloglucan endotransglycosylase/hydrolase (XTH) plays an important role in the structure and function of plant cell walls and is involved in osmotic stress responses | Populus tomentosa |