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Literature summary for 2.4.1.207 extracted from

  • Han, Y.; Ban, Q.; Li, H.; Hou, Y.; Jin, M.; Han, S.; Rao, J.
    DkXTH8, a novel xyloglucan endotransglucosylase/hydrolase in persimmon, alters cell wall structure and promotes leaf senescence and fruit postharvest softening (2016), Sci. Rep., 6, 39155 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
gene XTH8, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression in onion epidermal cells at the cell wall, recombinant overexpression of DkXTH8 in Arabidopsis thaliana and in Solanum lycopersicum Diospyros kaki

Protein Variants

Protein Variants Comment Organism
additional information recombinant overexpression of DkXTH8 in Arabidopsis thaliana and in Solanum lycopersicum, DkXTH8 expression causes cells to be easily destroyed, increases membrane permeability and cell peroxidation, and accelerates leaf senescence and fruit softening in transgenic plants Diospyros kaki

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall transient expression assays in onion epidermal cells indicate direct localization of DkXTH8 to the cell wall via its signal peptide Diospyros kaki 5618
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Organism

Organism UniProt Comment Textmining
Diospyros kaki A0A067YMX8 cv. Fupingjianshi, i.e. Diospyros chinensis
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Source Tissue

Source Tissue Comment Organism Textmining
calyx very low expression level Diospyros kaki
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flower very low expression level Diospyros kaki
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fruit ripe fruits, mainly Diospyros kaki
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leaf very low expression level Diospyros kaki
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additional information transcriptional profiling reveals that DkXTH8 peaks during dramatic fruit softening. Very lo expression levels in flowers, calyces, leaves and stems, respectively Diospyros kaki
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stem very low expression level Diospyros kaki
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information when expressed in vitro, the recombinant DkXTH8 protein exhibits strict xyloglucan endotransglycosylase activity (XET, EC 2.4.1.207), whereas no xyloglucan endohydrolase activity (XEH, EC 3.2.1.151) is observed Diospyros kaki ?
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Synonyms

Synonyms Comment Organism
DkXTH8
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 Diospyros kaki
XET
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 Diospyros kaki
xyloglucan endotransglucosylase
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 Diospyros kaki
xyloglucan endotransglucosylase/hydrolase
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 Diospyros kaki

Expression

Organism Comment Expression
Diospyros kaki expression of DkXTH8 is suppressed by gibberellic acid and 1-methylcyclopropene during storage at room temperature down
Diospyros kaki expression of DkXTH8 is stimulated by propylene and abscisic acid during storage at room temperature up

General Information

General Information Comment Organism
malfunction overexpression of DkXTH8 results in increased leaf senescence coupled with higher electrolyte leakage in Arabidopsis and faster fruit ripening and softening rates in tomato. Transgenic plants overexpressing DkXTH8 display more irregular and twisted cells due to cell wall restructuring, resulting in wider interstitial spaces with less compact cells. DkXTH8 expression causes cells to be easily destroyed, increases membrane permeability and cell peroxidation, and accelerates leaf senescence and fruit softening in transgenic plants Diospyros kaki
physiological function fruit softening is mainly associated with cell wall structural modifications, and members of the xyloglucan endotransglucosylase/hydrolase (XTH) family are key enzymes involved in cleaving and rejoining xyloglucan in the cell wall. Isozyme DkXTH8, a xyloglucan endotransglucosylase/hydrolase in persimmon, alters cell wall structure and promotes leaf senescence and fruit postharvest softening Diospyros kaki