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Literature summary for 2.4.1.207 extracted from

  • Olsen, S.; Krause, K.
    Activity of xyloglucan endotransglucosylases/hydrolases suggests a role during host invasion by the parasitic plant Cuscuta reflexa (2017), PLoS ONE, 12, e0176754 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.207

Inhibitors

Inhibitors Comment Organism Structure
Coomassie Brilliant Blue R250 i.e. BB-R250, the parasite shows no preference towards inhibitor-coated petioles or control petioles, readily starting to develop haustoria on any of them. Sectioning and microscopical analysis of infection sites, that have progressed to the mature stage, reveal that approximately 1/3 of haustoria on 5 mM BB-R250-coated petioles have not been able to grow into the host tissue Cuscuta reflexa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted and acts on the cell walls of the host plants Cuscuta reflexa
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Organism

Organism UniProt Comment Textmining
Cuscuta reflexa
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Source Tissue

Source Tissue Comment Organism Textmining
haustorium
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 Cuscuta reflexa
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additional information the level of xyloglucan endotransglucosylation (XET) activity peaks at the penetrating stage of Cuscuta reflexa on its host Pelargonium zonale Cuscuta reflexa
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information incorporation of sulforhodamine-labelled xyloglucan oligosaccharides (XyGO-SR) into xyloglucan Cuscuta reflexa ?
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 ?

Synonyms

Synonyms Comment Organism
XET
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 Cuscuta reflexa
XTH
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 Cuscuta reflexa
xyloglucan endotransglucosylase
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 Cuscuta reflexa
xyloglucan endotransglucosylases/hydrolase
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 Cuscuta reflexa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
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 assay at Cuscuta reflexa

General Information

General Information Comment Organism
physiological function the holoparasitic angiosperm Cuscuta develops haustoria that enable it to feed on other plants. Cell wall modifications seem to be required in order for the parasite to successfully infect a host, e.g. Pelargonium zonale, changes to xyloglucan through the activity of xyloglucan endotransglucosylases/hydrolases (XTHs) are essential. In vivo colocalization of XET activity and donor substrate demonstrate XET activity at the border between host (petioles) and parasite, endotransglucosylation of xyloglucan at the host-parasite interface Cuscuta reflexa