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Literature summary for 2.4.1.207 extracted from

  • Genovesi, V.; Fornale, S.; Fry, S.C.; Ruel, K.; Ferrer, P.; Encina, A.; Sonbol, F.M.; Bosch, J.; Puigdomenech, P.; Rigau, J.; Caparros-Ruiz, D.
    ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana (2008), J. Exp. Bot., 59, 875-889.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
gibberellin
-
Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
into the pET28a+ vector for transformation of Escherichia coli BL21 cells, into the pPICZalphaA vector for transformation of Pichia pastoris X-33 cells, into the pCAMBIA1302 and pCAMBIA1300 vectors for transformation of onion and Arabidopsis thaliana cells, respectively Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall ZmXTH1 is weakly bound to the cell wall Zea mays 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
-
Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Zea mays breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan ?
-
?

Organism

Organism UniProt Comment Textmining
Zea mays Q5JZX2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Zea mays

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-
root elongation zone of the maize root Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan Zea mays ?
-
?

Synonyms

Synonyms Comment Organism
xyloglucan endotransglucosylase/hydrolase
-
Zea mays
ZmXTH1 in addition to ZmXTH1 and Zm1005, six other XTH genes are identified in maize EST databases Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
enzymatic assay at room temperature Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
enzymatic assay Zea mays